Question

In a glucose limited chemostat, what happens to the cell density or cell yield of Enterobacter cloacae when 0.01% phenol is included in the reservoir medium?

Answer 1

A chemostat is a type of bioreactor to which medium is ceaselessly included, whereas culture media containing cleared out over supplements, metabolic conclusion items and microorganisms are persistently expelled at the same rate to keep the culture volume constant. By changing the rate with which medium is included to the microorganism can be effectively controlled inside limits. Cells at first developed in clump culture at high glucose concentration required long-term adjustment to nutrient limited development conditions within the chemostat (between 100-200 volume changes at D=0.6h) until steady-state with regard to leftover glucose concentration was come to; for adjusted cells, be that as it may, modern steady-state glucose concentrations were more often than not gotten inside less than 10 volume changes.

Generally phenol used as a pH indicator or buffer. If 0.01% phenol is included in the reservoir media, their will be not effect in cell yield, as it buffer which will control the pH of the growth media.