Question

so question, What are some drawbacks using intensive soil extract medium (ISEM)? based on a new soil extract using 80% methanol? it is used to isolate uncultured bacteria and new taxanomic canidates

Answer 1

Intensive soil extract medium ( ISEM ) based on new soil extract (NSE) using 80% methanol was used to efficiaently isolate previous uncultured bacteria and new taxonomic candidates , which announced for 49% and 55% of the total isolates examined ( n= 258) , respectively. The isolates were affiliated with seven phyla ( Proteobacteria , Acidobacteria , Firmicutes , Actinobacteria , Verrucomicrobia, Planctomycetes , and Bacteriodetes) . The drawbacks of this are NSE included more diverse components of low molecular weight organic substances than two conventional soil extracts made using distilled water. Cultivation of previously uncultured bacteria is expected to extend knowledge through discovery of new phenotypic , phisiological and functional properties and even roles of unknown genes . Importance either metagenomics or single-cell sequencing can detect unknown genes from uncultured microbial strains in environment and may find their potential metabolitis and roles. However , such gene/genome-based techniques do not allow detailed investigations that possible with culture. To solve this problem , various approaches for cultivation of uncultured bacteria have been developed , but there are still difficulties in mataining pure culture by subculture.