Question

6. Glycosylation involves the covalent addition of sugar moieties to specific amino acids, and is an important post-translational modification. Suggest challenges in proteomics analysis that might arise from glycosylation (and any ideas to address them).

Answer 1

Challenges in proteomics analysis that arise from glycosylation:

Proteomics is in large scale the study of proteins. A proteome is referred to the entire set of proteins that are generally produced by an organism or modified by a system. This protein glycosylation is considered to be one of the mst cmplex and difficult post transcriptional process. There are many challenges involved in the study of proteins. Some of the important challenges are:

Protein structures involve three types. They are primary, secondary and tertiary. The first and the main challenge is to maintain these structures during the complete process.There are few proteins which are very difficult to dissolve .Hence it is very difficult to analyse them. Proteins cannot be amplifies. Hence it is difficult to detect the less abundant species.

There are many technical obstacles to overcome before it is taken into a clinical stage. This is being addressed by standardization of methodologies and dissemination of proteomics data into databases which are publicly available. Most of these proteomics have complex instrumentation, critical computing and also expensive.

The major challenge will be the integration of this proteomics with the genomics and the metabolomics data. It is even difficult to identify and interpret the data with the clinical results and epidomology.