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You have about 0.5 ml of a crude protein extract. Your lab instructor has asked you...

You have about 0.5 ml of a crude protein extract. Your lab instructor has asked you to make a 1:100 dilution, but it is necessary to save most of your sample for another experiment. How would you go about making up 3.0 ml of the 1:100 dilution?

As luck would have it, you learn that your instructor gave you the wrong information in the above example and now he wants you to make a 1:135 dilution rather than a 1:100. You still have to save the remainder of your stock and therefore you must make the appropriate dilution from the 1:100 sample. What should you do?

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