make up 52 mL of a 1:10,000 dilution of the Sybr Green stock solution. Dilute the Sybr Green stock solution with TAE.
Dilution ratio is 1:10,000
Dilution ratio = the ratio of solute to solvent .
To dilute Sybr Green solution we have to use ,
C1V1 = C2V2
52×10,000 = 1 × V2
5,20,000 mL = V2 ( volume of TAE needed )
V2 = 520 Litres .
make up 52 mL of a 1:10,000 dilution of the Sybr Green stock solution. Dilute the...