Question

Consider a cell line with a mutation in a gene essential for the G₂/M Checkpoint. These cells do not pause at this checkpoint. Briefly describe one possible consequence of the mutation on cells moving through the cell cycle.

Answer 1

• There are specific protein which are responsible for the g2 m checkpoint such as p53 and p21.
• These proteins ensure that the damaged dna is not passed through mitosis.
• Proteins such as Rad3 and rad18 are responsible for the maintainance of the G2 checkpoint.
• So when normally the damaged dna passes through the checkpoint apoptosis occur.
• If some of the protein responsible for the checkpoint maintainance are mutated the damaged dna will pass through the checkpoint and cancer will occur.