2. Describe briefly these two techniques that are used to
amplify DNA fragments to obtain
genome sequences (around 100 words extension for each one).
a) Emulsion PCR
b) Bridge PCR
Emulsion pcr is used by Next generation Sequencing to replicate the DNA sequences.
It derives its name because it is conducted on a bead surface within tiny water bubbles on oil solution.
Basic principle is to dilution and compartmentalization of template molecules.
the dilution is to a degree where each droplet contains a single template molecule and functions as a micro-PCR reactor.
Steps involved:
Finally beads are placed on a slide and amplified.
BRIDGE PCR
Bridge PCR is a PCR technique that embeds DNA on a surface for cloning.
Steps involved
1. DNA is fragmented and adapters are ligated to both ends.
2. DNA is then denatured and then they attach to their corresponding adapter sequences.
3. dNTPs, and DNA polymerase enzyme is added to elongate DNA strands.
4. Denaturation the newly formed DNA strands and repeat until dense clusters are generated in each channel of flow cell. Until about millions DNA clusters is formed.
2. Describe briefly these two techniques that are used to amplify DNA fragments to obtain genome...