Question

1. Which of the following statements regarding eukaryotic transcription is true?

a. Different genes can be transcribed from either DNA strand

b. Multiple genes can be transcribed at one time

c. Transcription and translation are coupled together

d. The RNA polymerase binds directly to the promoter to initiate transcription

e. All regulatory elements are directly upstream, adjacent to the start site

2. Which of the following is false regarding elongation of transcription?

a. ribonucleosides are added to the 3'OH end of the RNA strand

b. RNA polymerase can backtrack and proofread

c. the same RNA polymearse is used in both prokaryotes and eukaroytes for protein-coding genes

d. RNA polymerase does not need a separate RNA primer like DNA polymerase does

e. None of the above

Answer 1

• A single gene can be acted upon by different RNA polymerases but not many genes by single RNA polymerase. Thus option b is incorrect.
• Transcription and translation are not  be coupled in eukaryotes because transcription takes place in nucleus and translation takes place in cytoplasm . So, option c is also incorrect.
• RNA polymerase binds to promoter along with certain transcription factors in the eukaryotes. So, option d is incorrect.
• Regulatory elements are present even 1000kb away from the transcription site. Hence option e is also incorrect.
• Both the strands in the DNA can be transcribed to produce different RNA thus different protein. Whichever strand can be  used as template strand and the opposite strand is non template strand.
• Hence option A is true.