Question

A cell culture with a concentration of 1.5x10^6 cells/ml has an absorbance at 600nm of 0.15. When the absorbance of the culture reaches 0.45 the cell concentration will be?

Answer 1

Answer: 4.5*10^6

Explanation : According to Beer lambert law A = a() * b * c, where A is the measured absorbance, a() is a wavelength-dependent absorptivity coefficient, b is the path length, and c is the analyte concentration.

Therefore, absorbance is directly proportional to cell concentration.