Question

1. Which of the following is (are) NOT true about ion exchange chromatography?

a. positively charged proteins elute first

b. negatively charged proteins elute first

c. uncharged proteins elute first

d. hydrophobic proteins elute first.

2. Which of the following is (are) true about hydrophobic interaction chromatography?

a. will be used to purify GFP

b. low salt buffer elutes captured proteins

c. the column is hydrophilic and the buffer is hydrophobic

d. the column is uncharged

3. If bacteria growing on the LB/amp plate were streaked on LB/amp/ara plates then

a. they would fluoresce immediately

b. they would not fluoresce because they do not have plasmid

c.they would die

d. they would not fluoresce but their daughter cells would fluoresce

e. they would fluoresce once gene expression occurred

Answer 1

Ans.1) Option C: uncharged proteins elute first &

Option D: hydrophobic proteins elute first.​

Theory: Ion exchange chromatography is a technique to separate charged or ionic molecules based on its affinity to ion exchanger material. Hence uncharged proteins and hydrophobic proteins do not elite using this method.