Question

Binding a ligand to a resin and then passing a protein extract solution over the resin to see which proteins bind to the ligand is an example of which of the following:

		Fluorescent resonance energy transfer
		Affinity chromatography
		Co-immunoprecipitation
		Equilibrium dialysis

The correlation of conserved loci with the importance of function of those loci is an example of which approach to functional genomics?

		Genetic approach
		Biochemical approach
		Evolutionary approach
		Functional approach

Answer 1

Answer =

Binding a ligand to a resin and then passing a protein extract solution over the resin to see which proteins bind to the ligand is an example of

Affinity chromatography

Explanation = Affinity chromatography is a technique used for the purification of proteins on the basis of affinity with ligands.there is highly specific interaction between protein and ligand.

in this technique the column is filled with resin , to that resin the ligand is attached , then a protein extract solution is passed over column ,then the protein has higher affinity with ligand is retained in the column while other are eluted out through column.

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