Question

Which of the following statements best describes the difference between a primary and secondary antibody as used in a Western blot? Choose one.

A. Primary antibodies are covalently bound to an antigen that the secondary antibody, with its conjugated enzyme, also recognizes.

B. The primary antibody and secondary antibody must be produced in the same animal.

C. Both antibodies bind and recognize antigen proteins; the secondary body is helped by the primary antibody.

D. The primary antibody binds the antigen, whereas the secondary antibody binds the primary antibody and serves to detect the complex with its linked enzyme.

Answer 1

D is correct answer because primary antibody bind on antigen and 2ndry antibody conjugated with enzyme bind on primary antibody and when substrate of enzyme is added , it help in detection with its linked enzyme.