Which of the following materials are NOT required to perform a PCR reaction? Select one: a. DNA polymerase b. RNA template c. DNA template d. free nucleotides e. two different primers
The correct answer is b. RNA template.
Explanation: Polymerase Chain Reaction (PCR) is an in vitro method that is used to amplify the target DNA sequences present within the DNA template.
Therefore, to perform a PCR reaction, DNA template, free nucleotides (dATP, dGTP, dCTP, and dTTP), DNA polymerase (the polymerizing enzyme), primers (initiate the synthesis of new DNA strands which are complementary to the template) are required.
So, RNA template is not required to perform the PCR reaction.
Which of the following materials are NOT required to perform a PCR reaction? Select one: a....