Question

What are the components of PAGE staining and destaining solution and write their functions too?

Answer 1

Answer

Components of PAGE staining solution ( Fix the gel by Methanol and acetic acid and G-250 dye binds with protein and colored them with blue color)

Coomassie brilliant Blue Dye (G-250) 0.1%

Methanol 50%

Glacial acetic acid 10%

Water 40 %

Components of the destaining solution ( excess dye binds with PAGE gel other than protein remove by the staining solution protein band ( Blue color) can be visible on the transparent background of the PAGE gel)

Methanol 50%

Glacial acetic acid 10%

Water 40 %