During a chromatography experiment, a student calculated two Rf values. They are A: .62 and B:...
Homework Answers
Rf = distance travelled by solute/distance travelled by solvent front
Distance travelled by A = Rf of A x distance travelled by solvent front
= 0.62 x 25.0 = 15.5 cm
Distance travelled by B = Rf of B x distance travelled by solvent front
= 0.30 x 25.0 = 7.5 cm
Distance between A and B = 15.5 - 7.5 = 8.0 cm
Add Answer to:
During a chromatography experiment, a student calculated two Rf values. They are A: .62 and B:...
1. List two errors in the typical student set up for performing thin layer chromatography illustrated...
1. List two errors in the typical student set up for performing thin layer chromatography illustrated here. 2. Define the following terms: Analyte: Stationary Phase: Mobile phase: Solvent Front: Origin: Rf Value: 3. Compound y traveled 6.2 cm while the solvent front traveled 11.1 cm. Calculate the Rf value for compound y. 4. Compound Z has an Rf value of 0725. When a chromatogram was developed, it moved 3.3 cm from the origin. How many cm did the solvent front...
2. The diagram below shows the results of a simple thin layer chromatography experiment. height reached...
2. The diagram below shows the results of a simple thin layer chromatography experiment. height reached by the solvent the "solvent font a) Describe briefly, but precisely, what you would have done in order to get to this stage. You can assume that you have been given a suitable thin layer chromatography plate. b) Why is there a cover on the beaker? c) In order to help identify the things in a chromatogram, you can measure the Rf value for...
Case C Thin Layer Chromatography 1. TOXI-LAB is a screening method that uses: chromatography (column /...
Case C Thin Layer Chromatography 1. TOXI-LAB is a screening method that uses: chromatography (column / planar) The first step consists of the extraction of drug metabolites from biological fluids when we place the sample in a test tube containing a mixture of solvents and buffering salts that cause the extraction of basic and neutral drugs. The solvent extract of the sample is concentrated by heat and evaporation, then is deposited onto a small disc of chromatographic media (small circle...
Example 3. Consider a Thin layer chromatography experiment of compounds A, B, C and D using...
Example 3. Consider a Thin layer chromatography experiment of compounds A, B, C and D using 50:50 Acetone Ether showed four spots under UV light (3 points) a) Calculate the Rf values for all spots A, B and C-show your work b) Match spots A, B, C and D with the four compounds below? solvent front A С В Но Но ОН ОН
Example 3. Consider a Thin layer chromatography experiment of compounds A, B, C and D using 50:50 Acetone : Eth...
Example 3. Consider a Thin layer chromatography experiment of compounds A, B, C and D using 50:50 Acetone : Ether showed four spots under UV light (3 points) a) Calculate the Rf values for all spots A, B and C-show your work b) Match spots A, B, C and D with the four compounds below? solvent front O Die A B C D OH
Describe the differences between paper chromatography and TLC. Give two advantages and two disadvantages of each...
Describe the differences between paper chromatography and TLC. Give two advantages and two disadvantages of each method for analysis. In order to prepare for a column chromatography separation of 3 ink dye components, you have run a TLC experiment with three possible solvent mixtures. These are the results: Acetone:Hexane in 1:1 ratio gave the following distances: solvent=4.2cm, pigment #1=3.8cm, pigment #2=1.7cm, and pigment #3=1.3cm Acetone:Hexane in 2:1 ratio gave the following distances: solvent=4.3cm, pigment #1=3.8cm, pigment #2=2.1cm, and pigment...
26. In a certain paper chromatography experiment, the Re values of components X, Y, and Z...
26. In a certain paper chromatography experiment, the Re values of components X, Y, and Z are 0.25, 0.50, and 0.75. If the spot of component Z traveled 72 mm, the solvent front would have traveled A. 18 mm B. 24 mm C. 54 mm D. 96 mm E. 216 mm 27. In the experiment described in question 26, the spot of X would have traveled A. 18 mm B. 24 mm C. 54 mm D. 96 mm E. 216...
2. If two different compounds have the same Rr value, how might they be identified using paper chromatography?...
2. If two different compounds have the same Rr value, how might they be identified using paper chromatography? 3. Why is it important to keep the spots on the paper as small as possible? 4. If the solvent pool at the bottom of the beaker touched the spots on the 1.5cm line, what would happen to the spots? How would you address this error? 5. If you were teaching a student how to do paper chromatography, what three pieces of...
Answer the following two questions A paper chromatography experiment was conducted on a mixture of amino...
Answer the following two questions A paper chromatography experiment was conducted on a mixture of amino acids including Aspartic acid (Asp), Glutamic acid (Glu), Histidine (His), Leucine (Leu), Phenylalanine (Phe) and Proline (Pro). The following Rf values were determined: Asp (0.245), Glutamic (0.275), His (0.295), Leu (0.745), Phe (0.685) and Pro (0.445). In the experiment, a colored spot for unknown amino acid "W" was found centered at a distance 25 mm from the start line when the solvent line had...
LOS SCULUI EXPERIMENT # 6 PAPER CHROMATOGRAPHY LABORATORY REPORT DATA AND CALCULATIONS Measure the retention factors...
LOS SCULUI EXPERIMENT # 6 PAPER CHROMATOGRAPHY LABORATORY REPORT DATA AND CALCULATIONS Measure the retention factors (R) for each of the components and report the values in the data table. Student Name Course Section Date Instructor PRE-LAB ASSIGNMENT son Rd? Hetardation factor Re Value) is the distance a given component (analute) divided by the distance the Re values o f mobile phase There is no shry unit for i migration distance of substance Imigrahon 1. What are the units on...
1. List two errors in the typical student set up for performing thin layer chromatography illustrated here. 2. Define the following terms: Analyte: Stationary Phase: Mobile phase: Solvent Front: Origin: Rf Value: 3. Compound y traveled 6.2 cm while the solvent front traveled 11.1 cm. Calculate the Rf value for compound y. 4. Compound Z has an Rf value of 0725. When a chromatogram was developed, it moved 3.3 cm from the origin. How many cm did the solvent front...
2. The diagram below shows the results of a simple thin layer chromatography experiment. height reached by the solvent the "solvent font a) Describe briefly, but precisely, what you would have done in order to get to this stage. You can assume that you have been given a suitable thin layer chromatography plate. b) Why is there a cover on the beaker? c) In order to help identify the things in a chromatogram, you can measure the Rf value for...
Case C Thin Layer Chromatography 1. TOXI-LAB is a screening method that uses: chromatography (column / planar) The first step consists of the extraction of drug metabolites from biological fluids when we place the sample in a test tube containing a mixture of solvents and buffering salts that cause the extraction of basic and neutral drugs. The solvent extract of the sample is concentrated by heat and evaporation, then is deposited onto a small disc of chromatographic media (small circle...
Example 3. Consider a Thin layer chromatography experiment of compounds A, B, C and D using 50:50 Acetone Ether showed four spots under UV light (3 points) a) Calculate the Rf values for all spots A, B and C-show your work b) Match spots A, B, C and D with the four compounds below? solvent front A С В Но Но ОН ОН
Example 3. Consider a Thin layer chromatography experiment of compounds A, B, C and D using 50:50 Acetone : Ether showed four spots under UV light (3 points) a) Calculate the Rf values for all spots A, B and C-show your work b) Match spots A, B, C and D with the four compounds below? solvent front O Die A B C D OH
26. In a certain paper chromatography experiment, the Re values of components X, Y, and Z are 0.25, 0.50, and 0.75. If the spot of component Z traveled 72 mm, the solvent front would have traveled A. 18 mm B. 24 mm C. 54 mm D. 96 mm E. 216 mm 27. In the experiment described in question 26, the spot of X would have traveled A. 18 mm B. 24 mm C. 54 mm D. 96 mm E. 216...
2. If two different compounds have the same Rr value, how might they be identified using paper chromatography? 3. Why is it important to keep the spots on the paper as small as possible? 4. If the solvent pool at the bottom of the beaker touched the spots on the 1.5cm line, what would happen to the spots? How would you address this error? 5. If you were teaching a student how to do paper chromatography, what three pieces of...
Answer the following two questions A paper chromatography experiment was conducted on a mixture of amino acids including Aspartic acid (Asp), Glutamic acid (Glu), Histidine (His), Leucine (Leu), Phenylalanine (Phe) and Proline (Pro). The following Rf values were determined: Asp (0.245), Glutamic (0.275), His (0.295), Leu (0.745), Phe (0.685) and Pro (0.445). In the experiment, a colored spot for unknown amino acid "W" was found centered at a distance 25 mm from the start line when the solvent line had...
LOS SCULUI EXPERIMENT # 6 PAPER CHROMATOGRAPHY LABORATORY REPORT DATA AND CALCULATIONS Measure the retention factors (R) for each of the components and report the values in the data table. Student Name Course Section Date Instructor PRE-LAB ASSIGNMENT son Rd? Hetardation factor Re Value) is the distance a given component (analute) divided by the distance the Re values o f mobile phase There is no shry unit for i migration distance of substance Imigrahon 1. What are the units on...
Most questions answered within 3 hours.
-
Where is the error in this code sequence?
String s1 = "Hello";
String s2 = "ello";...
asked 10 months ago -
Financial data for Joel de Paris, Inc., for last year
follow:
Joel de Paris, Inc.
Balance...
asked 10 months ago -
Consider this reaction:
Al2(SO4)3 (aq)+ BaCl3
(aq) Al2Cl6 (aq)- +
3BaSO4(s) . What is the...
asked 10 months ago -
Suppose that Savneet is considering increasing her
recent random sample from 20 car rentals to 40...
asked 10 months ago -
Trucks arrive at an unloading terminal at an average rate of 120
per hour.
Trucks arrive...
asked 10 months ago -
Why are methanol and ethanol completely soluble in water while
octanol is not very little soluble....
asked 10 months ago -
A facilities manager at a university reads in a research report
that the mean amount of...
asked 10 months ago -
When the CuSO4 is rehydrated by adding water to the anhydrous
compound, is this an endothermic...
asked 10 months ago -
A ray of sunlight is passing from diamond into crown glass; the
angle of incidence is...
asked 10 months ago -
A block of mass 0.249 kg is placed on top of a light, vertical
spring of...
asked 10 months ago -
how do the kidneys compensate in the presences of acidosis
a) trigger hyperventilate
b) reserve acid...
asked 10 months ago -
Question 501 pts
The rental rate of capital to the firm increases. Which of the
following...
asked 10 months ago