Question

Question: I need help to construct this I need help to construct this lab report ... I need a well explained introduction and basics methods.

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INTRODUCTION Introduce the overall idea behind our lab experiments using buccal cells

1. Real time PCR, using SYBR Green I dye

2. Genotype DNA SNP (warrior-worrier gene)

3. Genotype DNA for SNP (ACTN3 gene)

METHODS

1. Isolation and dilution of DNA: summarize the method used to extract and dilute your gDNA.

2. SYBR Green-based qPCR reaction: provide theoretical background on these techniques- and summarize the set up of master mix so that a student who never done it can understand what you have done and how to do it.

3. Taqman-based qPCR reactions: provide theoretical background on these techniques- and summarize the set up of master mix so that a student who never done it can understand what you have done and how to do it. Also highlight what the major change between Taqman and SYBR

Answer 1

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