Question

How can I do this problem step by step? How did they get the following answers?

SDS-PAGE Gel filtration in buffer Question 3 (9 pts) Referring to the data at the right, deduce the schematic structure of proteins A-C. At the right is the behavior of the8003 proteins on size exclusion chromatography Use the sample answer for the amount of detail 3a- required, show the quaternary structural arrangement including disulfide linkages (if appropriate) 230 <Da 60 kD 10 D Protein A Protein B Protein C 00 kDS 60 kDa 60 kDa 40 ADa 8 20 kDa 30 KDa 20 kDa Elution volume +/-β-mercaptoethanol Protein A Protein B Protein C Sample answer -5 20 kDa kDa 20 30 4 90 kDa Indicate disuifides where appropnate

Answer 1

a macep ethanol shoos kcnd 2ος 30 kDa . and 40 kDq 4o kDa 20 5 3o KDa subunits aue alfahed disuphide bond, ohi Protein B : 3 Protein Gel FilHanshouos proein C is 9o KDa protein and Pme Tcaptoethanoleamen gives band on S KDa point.