Question

1) Show the conversion between volume of stock and final concentration for one of the standards. Show all work and give the concentration and equations used.

2) Use the Lambert-Beer’s law equation to calculate the concentration of iron in your patient’s sample (Unknown B). Show all steps of your calculation.

Based on the calculations from your experimental results, is the iron level in your patient’s blood too low or too high?

3) Explain in detail how you would treat your patient’s condition. As an example, give the specific treatment for a 20.0 mL sample of blood. (“Increase iron level” or “decrease iron concentration” are not sufficient answers)

Data Table: Volume Stock Final Concentration Absorbance Initial Concentration 0.060 0.183 0.308 0.366 0.432 0.471 0.398 50 μg/dL 110 μg/dL 140 μg/dL 170 μg/dL 200 μg/dL 220 ug/dL Unknown B 10.00 mL 10.00 mL 10.00 mL 10.00 mL 10.00 mL 10.00 mL Show the conversion between volume of stock and final concentration for one of the standards. Show all work and give the concentration and equations used. Show all necessary calculations. e here to search

Answer 1

nene 、mitīap volume Finel ara O.39 8

Absorbance 0.06 0.183 0.308 0.366 0.432 0.471 Concentration 50 110 140 170 200 220 0.6 y 0.002x - 0.066 2 0.5 a) 0.4 e 0.3 <4 0.2 0.1 0 250 150 200 100 50 0 Concentration