Question

2. The structure of the solid phase Wang resin linker and the first amino acid that we will use in this lab is shown below Fmoc first amino acid linker a) (1 pt) If the resin has 0.72 mmol/g of this linker on the surface of the beads, and you use 300 mg of resin for the reaction, how many mmol of linker do you have? b) (3 pts) All peptides will use alanine at the C-tenminus (position 1), Fmoc-L-valine at position 2, Fmoc-L-aspartic acid 5-tert-butyl ester at position 3 and valine again at position 4. The final amino acid added to the N-terminus will vary - half the groups will add acetyl-o-tert-butyl-L-tyrosine and the N-acetyl-3,5-diiodotyrosine. Calculate how many grams of your amino acids that will be needed for the coupling steps. To ensure complete coupling of each amino acid to the resin, for each reaction you want to add 4 molar equivalents of your assigned amino acid relative to the number of moles of linker (multiply the value from (a) by 4) Fill in the table below with the information for your assigned peptide half other add Weight in grams Molecular weight corresponding to 4 molar (with Fmoc and relevant equivalents of this amino protecting groups) acid relative to the moles Amino Acid Name (see your assigned structure) of linker 1 (C-terminus Alanine (already on resin) Fmoc-L-valine 2 Fmoc-L-aspartic acid 5-tert-butyl ester 4 Fmoc-L-valine 5 (N-terminus) Tyrosine derivative Fmoc Protection Strategy To prevent amino acids from reacting with themselves, the amine- end is 'protected" with an fluorenylmethoxycarbonyl (Fmoc) group Fmoc-amino acid N H1 NH но но Fmoc' group .The carboxylic acid end can then be coupled to the growing peptide chain N H HO growing peptide chain on bead

Answer 1

male。 g 1.ruker enthesqaceg.tn_beache) 2-1 → 0。216 mm ol Pos ition Nome Moleculorwe A lanine 1 Fmoc-Lvaline 2. Fmoc-L t buty eoter 6 3-29 4t cuu9iNg.hrac-. L-valine 0,86LAX gg.l-) '85. 62.YA