A specimen sample was streaked in a plate of agar. Only gram negatives grew on the agar and certain gram negatives resul...
Homework Answers
CLED is a combination of Blood Agar and MacConkey agar combined. Major constituents of CLED agar are Lactose, Pancreatic digest of gelatin and casein, beef extract, cystine, pH indicator Bromothymol blue and Agar. Bromothymol Blue is the pH indicator to differentiate lactose fermenters from lactose-nonfermenters. Organisms capable of fermenting lactose will lower the pH and change colour of the medium from green to yellow.
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A specimen sample was streaked in a plate of agar. Only gram negatives grew on the agar and certain gram negatives resul...
How would you verify that the colonies that grew on a MacConkey agar plate were Gram...
How would you verify that the colonies that grew on a MacConkey agar plate were Gram negative? What types of bacteria are inhibited on MacConkey agar?
1)An eosin methylene blue (EMB) agar plate was inoculated with four different species of bacteria. Below...
1)An eosin methylene blue (EMB) agar plate was inoculated with four different species of bacteria. Below are the colony growth after inoculation. Which shows a vigorous lactose fermenter? A) A brown media with an oblong culture of bacteria. The colony shows vigorous growth and is the same color as the media. B) A brown media with an oblong colony of bacteria that experiences weak growth. The color of the media is visible through the translucent white color of the bacterial...
Background Background continued... Questions Experiment 2: TSI The triple sugar iron agar (TSI) can identify gram...
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Experiment 2: TSI The triple sugar iron agar (TSI) can identify gram negative bacteria based on how they metabolize carbohydrates. It contains three carbohydrates-glucose, lactose and sucrose, in addition to sodium thiosulfate which some bacteria use in the production of hydrogen sulfide (H2S). It also contains iron in the ferrous form and phenol red as a pH indicator. TSI is poured into a tube when heated to a liquid form, and the tube is slanted as...
Microbiology - Gram Positive (ONLY answer) [Q1] A positive catalase test shows - Bubbles - Appearance...
Microbiology - Gram Positive (ONLY answer) [Q1] A positive catalase test shows - Bubbles - Appearance of Clots - Change of color from blue to green. - All answers are correct. [Q2] A complete blood cell lysis is also known as - Alpha hemolysis - Beta hemolysis - Gamma hemolysis - No answer text provided. [Q3] How do you know whether your organism completely lysed the blood cells? - By the presence of a green halo around the bacterial growth...
Lab Report-Carbohydrates 1. Purpose 2. Special Media for Isolating Bacteria (Lab #12) a. Why are dyes...
Lab Report-Carbohydrates 1. Purpose 2. Special Media for Isolating Bacteria (Lab #12) a. Why are dyes such as phenol red, eosin or methylene blue added to the media? b. How does the bacterium change the media (i.e color of agar or colonies) after incubation? C. In this experiment, which media are selective, and which are differential? d. How did the results observe on the mannitol salt agar and EMB agar correlate to the Gram reaction of the bacteria? e. What...
You have obtained samples from a patient who is in the ICU at your hospital. You...
You have obtained samples from a patient who is in the ICU at your hospital. You perform the Gram-Stain and IMVIC tests, and see the following results: Gram-Stain Upon examination under the microscope you view pink colored rods. IMVIC Indole: After the addition of Kovac's Reagent, you see no color change at the top of the media. Methyl Red: After the addition of methyl red, your broth immediately turns a red color. Voges Proskauer: After the addition of VP reagents...
Q14. In the lab info it says that as a rule, most microbes can adjust to...
Q14. In the lab info it says that as a rule, most microbes can adjust to a range of salt concentrations. 7/29/2019 Which cell structure allows microbes to physically withstand hypotonic environments? Cell wall MSA has two properties. It is both selective and differential. When bacteria grow on this media, there is a visible difference between bacteria that ferment mannitol, and the bacteria that do not See Figure 2 (a)-effects of selective/differential media. Q15. Which of the following is correct...
microbiology help TOT Zoo Add Page Insert Table Chart Text Shape Media Comment These questions will...
microbiology help
TOT Zoo Add Page Insert Table Chart Text Shape Media Comment These questions will serve in lieu of a lab report for Exercise 15, 16, and 17 You will find the answer to these questions in the background, procedure, results and interpretation sections of manual Exercise 15, 16, and 17, videos, Actions of Selective and Differential Media Chart, and the Principle/Theory article in homework section.) General Questions 1. What is the purpose (function) of selective media? (How does...
1. You are a food scientist trying to determine the nutritional content of certain food samples....
1. You are a food scientist trying to determine the nutritional content of certain food samples. Foor food sample D you preform a Benedict test to see if it contains any monosaccharides. For this test, you take 3 droplets of food sample D and mix it with 2 droplets of the Benedict test solution. The solution is heated for 5 minutes and you observe the color of the solution changing from blue to green to orange. A color change from...
I. Solve the next problem You are asked to identify the cause of infection from a...
I. Solve the next problem You are asked to identify the cause of infection from a serious skin wound of a 60-year-old sailor involved in a ship fire. 50% of the patient's body presents second and third degree burn injuries. You are provided with a cotton swab with the patient's skin suppuration, which technique would you use to isolate all the cultivable bacterium infecting the wounds of the patient? Which type of medium would you use for this purpose? give...
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Background continued...
Questions
Experiment 2: TSI The triple sugar iron agar (TSI) can identify gram negative bacteria based on how they metabolize carbohydrates. It contains three carbohydrates-glucose, lactose and sucrose, in addition to sodium thiosulfate which some bacteria use in the production of hydrogen sulfide (H2S). It also contains iron in the ferrous form and phenol red as a pH indicator. TSI is poured into a tube when heated to a liquid form, and the tube is slanted as...
Lab Report-Carbohydrates 1. Purpose 2. Special Media for Isolating Bacteria (Lab #12) a. Why are dyes such as phenol red, eosin or methylene blue added to the media? b. How does the bacterium change the media (i.e color of agar or colonies) after incubation? C. In this experiment, which media are selective, and which are differential? d. How did the results observe on the mannitol salt agar and EMB agar correlate to the Gram reaction of the bacteria? e. What...
You have obtained samples from a patient who is in the ICU at your hospital. You perform the Gram-Stain and IMVIC tests, and see the following results: Gram-Stain Upon examination under the microscope you view pink colored rods. IMVIC Indole: After the addition of Kovac's Reagent, you see no color change at the top of the media. Methyl Red: After the addition of methyl red, your broth immediately turns a red color. Voges Proskauer: After the addition of VP reagents...
Q14. In the lab info it says that as a rule, most microbes can adjust to a range of salt concentrations. 7/29/2019 Which cell structure allows microbes to physically withstand hypotonic environments? Cell wall MSA has two properties. It is both selective and differential. When bacteria grow on this media, there is a visible difference between bacteria that ferment mannitol, and the bacteria that do not See Figure 2 (a)-effects of selective/differential media. Q15. Which of the following is correct...
microbiology help
TOT Zoo Add Page Insert Table Chart Text Shape Media Comment These questions will serve in lieu of a lab report for Exercise 15, 16, and 17 You will find the answer to these questions in the background, procedure, results and interpretation sections of manual Exercise 15, 16, and 17, videos, Actions of Selective and Differential Media Chart, and the Principle/Theory article in homework section.) General Questions 1. What is the purpose (function) of selective media? (How does...
I. Solve the next problem You are asked to identify the cause of infection from a serious skin wound of a 60-year-old sailor involved in a ship fire. 50% of the patient's body presents second and third degree burn injuries. You are provided with a cotton swab with the patient's skin suppuration, which technique would you use to isolate all the cultivable bacterium infecting the wounds of the patient? Which type of medium would you use for this purpose? give...
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