Question

how did they get these answers?

we Vulins Hemoglobin/Am 1. An allosteric interaction between a) binding of a molecule to a bir 0 You measure the initial rate of an enzyme reaction as a function of substrate concentration in the presence and absence of an inhibitor. The following data are obtained: [S] Vo -Inhibitor +Inhibitor 17 29 0.0001 0.0002 0.0005 0.001 0.002 0.005 0.01 0.02 0.05 0.1 100 100 100 98 99 100 100 0.2 a) What is the Vmax in the absence of inhibitor? b) What is the km in the absence of inhibitor? c) When [S] -0.0004, what will Vo be in the absence of inhibitor? d) When [S] -0.0004, what will Vo be in the presence of inhibitor? e) What kind of inhibitor is it likely to be? a) 100 60.0002 c) 66.7 d) 40 e competitive 100 b)

Answer 1

Solution: The kinetics of of enzymatic reactions can be ribed by the Michaelis-Menten equation, That is Vo = Vmax * is 1+ km [s] - Where vo. is the neaction nate ire [s]- is the substrate Concentration. Km is the halfo Saturation Constant. (also known as Michaelic Constant) mex - Maximum rate of the reaction. of the given following data, the absence of inhibiton Vmax ie maximum nate of the neaction is equal to 10o.

given b) from the data table; we Calculate km Vo - Vmax. [5] Drenthe [s]+ km Cad go ar 33 = 1 cox - 0.0001 + KM 0.0033 + 33 km = 0.01 33km = 0.0067 n a a km = 0.0002. adidad ei to The absence of inhibitor Kim is 0.0002. 9 e [s] = 0.0004 Vo ? (In absence of Inhibitor) Using the equation (1); we get 0.0004 Vo = 100 x - 0.0004 + 0.0002 0.04 0.0006 = 66.67 . . Vo = 66. 6 7 in the absence of inhibiton. d) Carlculate from the given data table we com Km is presence of inhibiton. Using equation -(1); we get- 17 = 100 x 0.0001 o ovol+ km → 0.0017 +17 km = 0.01 > 17km = 0.0083 → km = 0.00048 8.

( In Presence of Inhibiton) [s] = 0.0004 ; Vo =) Using equation ④ ; we get- ie 0.0004 Vo = 100x - 0.0004 + 0. ovoy88 0.oooeee = 45 Vo=45; In Presence of inhibiton sono es In Present of inhibitor the subsface Concentraction increases, therefore the michaelic Constant (km) also increases .so, Raises km value only. Hence, It's Competitive Inhibiton..