Question

In biotechnology, several sources of carbon are available to grow cells on. In order to minimize the waste of resources, it is important to be able to utilize other carbohydrates than glucose.

Xylose is one of the most abundant building blocks in hemicellulose and thus represents a large fraction of plant products. The yeast Saccharomyces cerevisiae cannot utilize xylose as part of its carbohydrate metabolism. Through mutagenesis, a xylose utilizing yeast mutant was established. This mutant was able to grow with xylose as the sole carbon source, and this ability could be attributed to the following enzymes that was active in the mutant yeast.

Xylose reductase (XR):

Xylose + NADH + H⁺→ Xylitol + NAD⁺

Xylitol dehydrogenase (XDH):

                      Xylitol + NAD⁺→ xylulose + NADH + H⁺

Xylulokinase(XK):

                      xylulose + ATP  → xylulose 5-phosphate + ADP + H⁺

^c) Explain briefly how products from the first relevant enzyme in B) can fully oxidized to CO₂at aerobic conditions. Your answer should specify ca. 4 key intermediates and names of three responsible biochemical pathways.

Answer 1

Hi please find the answer in the attached picture below.

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