Question

a) For the lac operon, will the repressor or RNA polymerase be bound to the operon in this situation? Draw what will be happening on the operon below. PROMOTER OPERATOR Lactose Enzyme 1 Lsctose Enzyme2 Lactose Enzyme 3 NO b) Will transcription occur? c) Describe what is happening (with vocabulary words). YES 2. Bobby Joe is fasting today, how will the E. coli in her stomach respond to the lack of Tryptophan? a) For the trp operon, will the repressor or RNA polymerase be bound to the operon in this situation? Draw what will be happening on the operon below. ROMOTER OPERATOR Tryptophan Enzyme 1 Tryptophan Enzyme 2 Tryptophan Enzyme 3 b) Will transcription occur? c) Describe what is happening (with vocabulary words). YES NO

Answer 1

Answer of question 2a

RNA polymerase would be bound to operon. But not the repressor. Image is attached.

Answer of question 2b

Yes, transcription will occur . Described in image and in answer of question 2c

Answer of question 2c

In Trp operon Repressor protein bound to operator and in inhibit binding of RNA polymerase. But repreasor protein in fully activated by CO-Repressor which tryptophan which is deficient in present situation. Below the step by step description of attached image

1) deficiency of Tryptophan (co-repressor)

2) so repressor protein is inactive

3) do not bind with operon

4) so repressor can not inhibit the binding pf RNA Polymerase with Operon.

5) RNA Polymerase binding intiate Transcription which results in mRNA synthesis

6) mRNA translated to tryptophan enzyme 1, 2 and 3

7) these enzyme synthesise tryptophan.

Answer to question 2b. Yes. It is describe above.

Answer of question 2c