Question

C370S19HW3 Name Please attach your plots to this sheet and turn in your work on Monday June 3 The enzyme urease catalyzes the hydrolysis of urea. 1. (3) Write the reaction catalyzed by Urea below: 2. (2) Of the 7 classes of Enzymes, which type is urease? 3. The rate of this reaction was determined for a series of solutions in which the concentration of urea was changed while maintaining a fixed urease concentration of 3.0 HM. The following data were obtained. 0.100 0.200 0.300 0.400 0.500 0.600 0.700 0.800 0.900 1.00 6.25 11.5 18.8 24.0 31.2 37.5 43.7 51.0 56.2 63.5 Determine the values of Vmax, k2, and Km for urease. You should create two plots 4. (4, see below for breakdown) The Michaelis Menten Plot rate vs [S] 5. (4, see below for breakdown) The Lineweaver Burke plot: 1/rate vs 1S I'm totally ok with you collaborating to complete this but EACH of you must turn in your own plots. Give your plot a title which includes your name (1). Label the axes appropriately.(2) Maximize your plot so that it fills the page(1) 6. For the M-M plot, estimate the Vmax, and the KM, marking them on your plot. (2) 7. For the L-B plot, Include a linear best fit equation for the L-B plot. (2). C370S19HW3 Name Please attach your plots to this sheet and turn in your work on Monday June 3 Using the L-B plot, show your work for 8 (3) Vmax 9. (3) k2 10. (3) Km 11. Urease is a metalloenzyme; this. Means that it has a metal cofactor. Go to the pdb and look up the structure 1E9Z. This is the crystal structure of urease from Helicobactor pylori, a common gut bacteria linked to ulcers and stomach cancer. a. (4)Describe the 1° structure b. (4)Describe the 2° structure C370S19HW3 Name Please attach your plots to this sheet and turn in your work on Monday June 3. c. (4)Describe the 3 structure d. (4) Describe the 4 structure e. (4)Describe the cofactor, i.e what metal and how is it attached to the protein? f. (4)Sketch the active site (the metal site) C370S19HW3 Name Please attach your plots to this sheet and turn in your work on Monday June 3 12. (5)Urease can be inhibited by Ni2 ions. Data is shown below. Based on this data, what is the mechanism of inhibition of urease by Ni. Explain. 050 200 20 b) S. mM

Answer 1

uneg A-NH ト (a) Ureaseis q