Question

4. If Bacterium X has a generation time of 60 minutes and 10 Bacteria X cells are inoculated into broth, how many hours will it be before there are 10° Bacteria Y colls in the broth culture? You MUST SHOW YOUR WORK to get credit. (2 % pts. total) 5. Using the graph below, draw the expected growth curve for a bacterial microorganism that spends 60 minutes in the lag phase, undergoes five (5) rounds of replication (generation time of 60 minutes), spends 120 minutes in stationary phase and then completely dies over the duration of the time shown on the graph. Start your graph at the black box at Time 0 minutes. (Assume that the relative measure of growth at this point is 8). Indicate the relative measure of growth (i.e., provide a number on the y-axis) at the end of exponential growth. (2 % pts . total). 10000- 1000- 100- 10- 1- 60 120 180 240 300 360 420 480 540 800 0 Time (minutes) Growth

please answer both questions and show necessary work

Answer 1

6enerahon Tne 60 mnutes Time talken by the bartena o double in Number dunng a specifecl tme penocl IniNal inoulation 1O bactenas = B inal inowa hon 10 battenas b 6=thme No Of generakons (n) b 6x 2n SOlue for n 2 n log 8 log logb los2 109 g lo3 y 2 no 19. 93 G nt 19.94ours 196 mnutes 60 minutes. 19.93 tme

t 6=60 minutes no S (me dunng expanen thal grouth) Then calwlate t t: Go minutes 300 minute x S = being bNo0f batenca at the endof exponen thal growth be Bx2 At the end Of the exponential growth b 8 x 25 256 Do0o Statonany Puare 256 -.. Decth Phafe Exponential Phare Lag Phase 300 SUO 60D 120 180 240 420 480 60 360 60+300 360+ 120