Question

4. Predict the TLC behavior to be expected from the three Anacin components (aspirin, caffeine, and 1,4-dimethoxybenzene). Assume you spot a plate with three standard solutions, each containing one of the components. Sketch what you expect the developed TLC plate to look like and identify which compound is which spot. 5. Draw a picture of the test tube with the prepared solution of the model compound and the prepared buffer and the beam of the UV spectrometer beam passing through it. Label each layer and identify which layer the beam is passing through. Hint: Refer to the answer to question 3 and note that the spectrometer beam passes through the bottom third of the test tube.

Answer 1

TLC The Butlern will look like as follows solvent front 1,4-dimethoxybenzene (Most Non-pelas) caffeine (ress pelar than aspisin) Aspirin e 1,2,3 represents three different selection Even if we do a co-spet it will look as follows. selvent font H 1,4-dimethoxybenzene & caffeine Aspirin y Combination of 1,243

Tre Anacin Campanus (aprin, caffeine and W-dimethoxybenzene) The structures for the above mentind Aspirin -Revelac) confunde an as followed Polering voi ly with harder of Polenty is Aspirin Caffite > Lydmanther L>2>3 2. Coffine ; beneane 3. 1,4-dimethoxydenzene : So order of Rf 3> 271 het Aspirin de agnato of t. time despite 2 14-dimethylten. Eine deagaty w 3. • The suutime of aboue metined writunes 1, 2 & 3 will share Retention factor Value defending up their falerity. The compound with highet polarity will thout a lower Rp vahie Impue up to a traller Blanding distance Conto! and the compound with buurt falrity will shue a higher Rf value and more up to a larger distance in a descending distance Bettom to top Rp value depends upon the palar nature of comprend by which they interact with my he a rt Aspirin will the most teher & has 38 Re Value beaux of 2 dipole-disele forces and one tysdagen bond, Then Caffiene will have teve falar native than aspirin & ocupy the and prition Rf value as it has cepability to inize and forma hydrogen bond with love furs of electrons on Nitrogen 1,4-dimethany benure will be non-faler due to presence of two identical mething groups