Question

Biochemistry: Concepts and Connections, 2e (Appling et al.) Chapter 8 Enzymes Set 2 1) Assume that an enzyme has the following kinetic constants: Vmax = 50 units/mg enzyme Km = 0.001M Km=0.001M D O Calculate the substrate concentration that will yield a reaction rate of a) 0.5 Vmax b) 0.33 Vmax

i know the answers a)0.001M and b)0.0005

Answer 1

Vmax S] km+ [S] Vo Vmax ES km Vo km Venax Cs 50 Vmax kmo.ool @ Vo = o-5 Vmax. oo m*x 0-5 Vmax 0-55כאo o. oo 35 50 50 LSJ 0-0olx1250 [S] = [S]=o.001x12s0 15ण

Vo 0.33 Vmax Km VmaxS 0.Eo1 50[S] 0-33XVMAX Vrmax 0-O0 50 LSJ O-33X50 tes 50 LS] OOol 50 [S] 33.5 825 = 0.00l X5 3 3.550 [S] [S] o-825 1675 [S]0.00 o 49 N O.OOo 5 [S]