Which of the following best describes an experimental design that would allow a researcher to use...
Which of the following best describes an experimental design that would allow a researcher to use the CRISPR/Cas9 system to increase the expression of a target gene? Assume the researcher adds the appropriate guide RNA in each case.
Select one:
a. expression of a catalytically inactive Cas9 enzyme fused to a HAT
b. expression of a catalytically active Cas9 enzyme fused to a HAT
c. expression of a catalytically active Cas9 enzyme fused to an HDAC
d. expression of a catalytically inactive Cas9 enzyme fused to a DNA Methyltransferase
Homework Answers
CRISPR is having two parts. One is guide RNA and other is CAS9 which is a nuclease enzyme. This enzyme break the nucleotide and make them inactive. HDAC is histone decatyltransferase enzyme that make the gene expression inactive. Therefore, cutting of this gene sequence will lead to the continuous gene expression. Hence, option c is correct.
Add Answer to:
Which of the following best describes an experimental design that would allow a researcher to use...
You wish to edit a gene in a population of stem cells using CRISPR/Cas9. You design...
You wish to edit a gene in a population of stem cells using CRISPR/Cas9. You design a plasmid and transfect it into the cells. Your plasmid included the guide RNA sequence with promoter, the Cas9 gene inserted between an appropriate promoter and termination sequence, and the usual Origin of Replication and Resistance/Marker gene. However, instead of being edited, your target gene is silenced and its protein is not produced at all. What is the most likely explanation? The cells have...
Please have a different answer than the other post, and use layman's terms as well hhmi...
Please have a different answer than the other post, and use
layman's terms as well
hhmi Biolnteractive Using CRISPR to identify the Functions of Butterfly Genes Activity Student Handout This document is made available by the Howard Hughes Medical Institute. Using this document, you agree to use this document in accordance with the Terms of Use. INTRODUCTION Scientists have determined the complete DNA sequences of the genomes for many organisms, including humans. By analyzing patterns in those sequences, they can...
<Problem Set 9a Question 4 4 of 5 > Part A - TUA: Uverview UUR HIDRL...
<Problem Set 9a Question 4 4 of 5 > Part A - TUA: Uverview UUR HIDRL yur g pruun When scientists use CRISPR-Cas9 to make specific cuts to a genome, they have a goal they're trying to achieve. Ohen, they either want to modify the protein that a particular gene encodes, or they want to block production of a particular protein. Depending on the goal, they customize the SANA they insert into the cell. For the CRISPR-Cas9 system to modify...
the following scenarios require application of CRISPR-cas9 technology. Identify which technologies are the best fit for...
the following scenarios require application of CRISPR-cas9
technology. Identify which technologies are the best fit for each
research scenario
Cas9 and HDR 1. Researchers want to make an endonuclease that binds to target DNA, but does not cut the DNA. This will allow them to target specific areas of the genome for other modifications without making cuts. dCas9 dCas9 and repression domain 2. Researchers are studying a proposed oncogene in mice. They want to examine what happens when the gene...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving rise to a “hairless” phenotype. In the homozygous condition, H is lethal. An independently assorting dominant allele S has no effect on bristle number except in the presence of H, in which case a single dose of S suppresses the hairless phenotype, thus restoring the "hairy" phenotype. However, S also is lethal in the homozygous (S/S) condition. What ratio of hairy to hairless flies...
1. Which of the following are the sites within the human body where carbon dioxide and...
1. Which of the following are the sites within the human body where carbon dioxide and oxygen are exchanged? A. Alveoli B. Arteries C. Synapses D. Venules 2. Which of the following describes the most important reason for repeating an experimental investigation? A. To verify the validity of the original findings B. To expand upon the original investigation C. To manipulate the independent variable D. To attempt to disprove the hypothesis 3. Lithium has an atomic number of 3 and...
1. According to the paper, what does lactate dehydrogenase (LDH) do and what does it allow...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
You wish to edit a gene in a population of stem cells using CRISPR/Cas9. You design a plasmid and transfect it into the cells. Your plasmid included the guide RNA sequence with promoter, the Cas9 gene inserted between an appropriate promoter and termination sequence, and the usual Origin of Replication and Resistance/Marker gene. However, instead of being edited, your target gene is silenced and its protein is not produced at all. What is the most likely explanation? The cells have...
Please have a different answer than the other post, and use
layman's terms as well
hhmi Biolnteractive Using CRISPR to identify the Functions of Butterfly Genes Activity Student Handout This document is made available by the Howard Hughes Medical Institute. Using this document, you agree to use this document in accordance with the Terms of Use. INTRODUCTION Scientists have determined the complete DNA sequences of the genomes for many organisms, including humans. By analyzing patterns in those sequences, they can...
<Problem Set 9a Question 4 4 of 5 > Part A - TUA: Uverview UUR HIDRL yur g pruun When scientists use CRISPR-Cas9 to make specific cuts to a genome, they have a goal they're trying to achieve. Ohen, they either want to modify the protein that a particular gene encodes, or they want to block production of a particular protein. Depending on the goal, they customize the SANA they insert into the cell. For the CRISPR-Cas9 system to modify...
the following scenarios require application of CRISPR-cas9
technology. Identify which technologies are the best fit for each
research scenario
Cas9 and HDR 1. Researchers want to make an endonuclease that binds to target DNA, but does not cut the DNA. This will allow them to target specific areas of the genome for other modifications without making cuts. dCas9 dCas9 and repression domain 2. Researchers are studying a proposed oncogene in mice. They want to examine what happens when the gene...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
Most questions answered within 3 hours.
-
Where is the error in this code sequence?
String s1 = "Hello";
String s2 = "ello";...
asked 11 months ago -
Financial data for Joel de Paris, Inc., for last year
follow:
Joel de Paris, Inc.
Balance...
asked 11 months ago -
Consider this reaction:
Al2(SO4)3 (aq)+ BaCl3
(aq) Al2Cl6 (aq)- +
3BaSO4(s) . What is the...
asked 11 months ago -
Suppose that Savneet is considering increasing her
recent random sample from 20 car rentals to 40...
asked 11 months ago -
Trucks arrive at an unloading terminal at an average rate of 120
per hour.
Trucks arrive...
asked 11 months ago -
Why are methanol and ethanol completely soluble in water while
octanol is not very little soluble....
asked 11 months ago -
A facilities manager at a university reads in a research report
that the mean amount of...
asked 11 months ago -
When the CuSO4 is rehydrated by adding water to the anhydrous
compound, is this an endothermic...
asked 11 months ago -
A ray of sunlight is passing from diamond into crown glass; the
angle of incidence is...
asked 11 months ago -
A block of mass 0.249 kg is placed on top of a light, vertical
spring of...
asked 11 months ago -
how do the kidneys compensate in the presences of acidosis
a) trigger hyperventilate
b) reserve acid...
asked 11 months ago -
Question 501 pts
The rental rate of capital to the firm increases. Which of the
following...
asked 11 months ago