Question

what are the advantages of reverse phase liquid chromatography?

Answer 1

Reverse phase offers many options to a chromatographer, but it depends upon us, detection method and what type of separation we are trying to achieve. There are many advantages of reverse phase over normal.

1. Reverse phase uses different types of columns which has hydrophobic stationary phase. Which means we can use water as mobile phase which is not possible in normal phase.

2. As we can use water as mobile phase, reverse phase can be used for biological research.

3. Reverse phase liquid chromatography allows use of pH selectivity to improve separations.

4. Hydrophobic solvents has differences in compressibility and UV cutoff which makes gradient separation tough. But we can use less hydrophobic or water in reverse phase to overcome these problems.

5.We can use water or less polar solvents as MeCN or MeOH, with highly controllable concentrations.

6. Columns are inexpensive and disposable.