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The catalytic efficiency of many enzymes depends o

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Answer #1

The change in Kcat between pH 6 and pH 7 must involve loss of a proton in the active

site. The best candidate is His-57. The increase in KM at higher pH must involve a change

in the binding site. The group involved is probably the N-terminus at lie-16, created by the cleavage that activates chymotrypsin.

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