Question

A good enzyme for use in an immunoassay should have a:

A. rapid degradation rate.

B. high substrate conversion rate.

C. high cost.

D. toxic enzymatic product.

What is the basis of strand displacement amplification?

A. Two different primers bind to the same strand of DNA.

B. Two different primers each bind to complementary strands of DNA.

C. Multiple primers bind to fragmented DNA targets.

D. Primers are joined together.

For a probe to hybridize to the target, which condition must be met?

A. The target must be double-stranded.

B. The target can only be RNA.

C. The target must have the same bases.

D. The target must have complementary bases.

Select the reaction characterized by amplification of a specific region of a DNA strand through denaturing and annealing.

A. Signal amplification

B. Polymerase chain reaction

C. Solution hybridization

D. Ligase chain reaction

How would you interpret results of DNA restriction analysis by gel electrophoresis if there was a difference in one band?

A. The samples are the same.

B. The samples are not identical.

C. Gel electrophoresis should be repeated.

D. The wrong buffer was used in electrophoresis.

In which technique is the signal amplified instead of the target DNA?

A. Branched DNA amplification

B. Polymerase chain reaction

C. Transcription-mediated amplification

D. Solution hybridization

Use of microarrays consisting of either DNA or RNA to identify an unknown nucleic acid best describes:

A. in situ hybridization.

B. polymerase chain reaction.

C. DNA chip technology.

D. strand displacement amplification.

In DNA sequencing, what is the purpose of the dideoxynucleotide bases?

A. To enhance synthesis of DNA

B. To stop growth of a DNA chain at a certain point

C. To make certain that only one strand of DNA is amplified

D. To create restriction fragment length polymorphisms

Answer 1

Enzyme in immunoessay should have - b, high substrate conversion rates

Basis of strand displacement amplification - b in strand displacement amplification two primer bind to complementary strands at opposite ends.

For a probe to hybridize, condition must be - d, target must have complementary bases, then only probe can binds to target

Reaction characterized by amplification of specific region of DNA by annealing and denaturation is - b

(polymerase chain reaction) it has three steps , denaturation, annealing, extension

Interpret results of DNA restriction analysis by gel electrophoresis, if difference in one strand -

C , gel electrophoresis should be repeated for both strand ,if difference occurs in band

Technique in which signal amplified instead of target DNA - b, polymerase chain reaction, in which amplification is done.

Use of microarrays consisting of either DNA or RNA to identify an unknown nucleic acid best describes - C. , DNA chip technology

In DNA sequencing, purpose of dideoxynucleotide bases - B, stop growth of DNA chain at certain point