1) What does PCR stand for and what does it do?
a. Polymerase Chain Reaction; PCR deletes DNA
b. Polymerase Copying Repeats; PCR amplifies DNA
c. Polymerase Copying Releats; PCR deletes DNA
d. Polymerase Chain Reaction; PCR amplifies DNA
2) During gel electrophoresis, the DNA fragments are separated by ____
a. charge
b. DNA fragments cannot be separated
c. color
d. size
3) Primers are
a. double stranded DNA oligonucleotide (fragment)
b. double stranded RNA oligonucleotide (fragment)
c. single stranded RNA oligonucleotide (fragment)
d. single stranded DNA oligonucleotide (fragment)
4)One of the differences between DNA Replication and PCR is the way the DNA double helix is denatured (open). DNA Replication uses an DNA-Helicase and PCR uses a. heat b. Ligase c. RNA-polymersase d. DNA-polymerase
5) Besides DNA from a biological sample, wWhat ingredients are needed for PCR
a. only primers
b. primers, the four nucleotides, and DNA polymerase
c. primers, the four nucleotides, DNA polymerase, and heat
d. primers and the four nucleotides
1. Option ''D'' is the correct answer.
PCR stands for polymerase chain reaction.This method is widely used in molecular biology.
Using PCR, copies of DNA sequences are exponentially amplified to generate thousands to millions of more copies of that particular DNA segment.
2. During gel electrophoresis, the DNA fragments are separated by size.
It is a laboratory method. It is used to separate mixtures of DNA,RNA, or proteins according to molecular size.
So option ''D'' is the correct answer.
1) What does PCR stand for and what does it do? a. Polymerase Chain Reaction; PCR...
Which of the following is the correct order of events in the polymerase chain reaction (PCR)? See Section 20.2 (Page 468) cooling to allow primers to attach; heating to separate double-stranded DNA; elongation of DNA strand as nucleotides are added elongation of the DNA strand as nucleotides are added; cooling to allow primers to attach; heating to separate double-stranded DNA heating to separate double-stranded DNA; cooling to allow primers to attach; elongation of DNA strand as nucleotides are added heating...
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used in laboratories was facilitated by the discovery of a
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Yellowstone National Park, in Wyoming. This organism contains a
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polymerase, which continues to function even after it has been
heated to 95°C.
a.Why would such a heat-stable polymerase be beneficial in
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b.What would happen if it weren’t
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