Homework Answers
DNA Polymerase: DNA polymerase catalyses the synthesis of DNA , it synthesizes a new strand of DNA on a DNA template. There are five different types of DNA polymerase I, Ii, Iii, IV, V.
DNA polymerase III are primarily involved in replication. It has four components I. e. Catalytic core, Dimerisation component, processivity component, clamp holder.
Primase: The enzyme primase synthesizes short RNA primers (<15 nucleotides) to prime DNA elongation.
SSB's : The SSB protein binds to the single strands to prevent subsequent reannealing.
DNA ligase : The joining of Okazaki fragments is done by enzyme ligase by catalysing the ends of the two DNA chains.
DNA Helicase : It opens up the DNA duplex at the replication fork to provide a single stranded template.
DNA gyrase: DNA gyrase removes the positive supercoils from the DNA during unwinding and introduce negative supercoils into the DNA.
DNA Helicase : it opens up the replication fork to provide a single stranded template
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D Question 1 (Matching) Match the enzyme/structure with its role in DNA replication. DNA polymerase synthesizes...
Which of the following statements about DNA replication is FALSE? Primase synthesizes the primers. DNA polymerase...
Which of the following statements about DNA replication is FALSE? Primase synthesizes the primers. DNA polymerase is required to add new nucleotides to the growing ends of the DNA strands. DNA ligase joins the small DNA fragments of the lagging strand. Only one strand of the parent DNA serves as a template for a newly synthesized complementary strand.
Match the component of DNA replication with its best description. 1- Beta clamp ................ 2- primer.....................
Match the component of DNA replication with its best description. 1- Beta clamp ................ 2- primer.................. 3- Helicase................ 4- Topoisomerase................... 5- DNA polymerase I...................... 6- DNA polymerase III...................... 7- primase...................... 8- Ligase...................... 9- SSBP...................... 10- Telomerase...................... 11- Okazaki fragment ...................... Description: - Replaces RNA - Break and rejoin covalent bonds - Provides a 3' OH for DNA polymerase III - Increases polymerase processivity - Is an RNA polymerase - Synthesizes leading strand and Okazaki fragments ...
Matching the normal function to the enzyme. This enzyme connects Okazaki fragments by forming bonds in...
Matching the normal function to the enzyme. This enzyme connects Okazaki fragments by forming bonds in the A. DNA backbone. Topoisomerase This enzyme opens the DNA helix, separating the strands to expose B. the bases. Primase This enzyme relaxes supercoils in the DNA that form ahead of the replication fork Helicase DNA Polymerase III This enzyme removes primers by cutting out one RNA nucleotide at a D. time and replacing it with DNA. DNA Polymerase This enzyme uses its own...
unwinds the dna double helix, synthesizes a short complementary piece of RNA primer, adds complementary nucleotides...
unwinds the dna double helix, synthesizes a short complementary
piece of RNA primer, adds complementary nucleotides in 5' to 3'
direction, forms covalent bonds between Okazaki
fragments
Primase [Choose ] DNA polymerase [Choose] DNA ligase [Choose] Helicase [Choose ]
Describe the process of DNA replication. (Your answer should include the following: replication fork, semiconservative replication,...
Describe the process of DNA replication. (Your answer should include the following: replication fork, semiconservative replication, replication fork, DNA gyrase, helicase, primase, DNA polymerase, DNA ligase, leading strand, lagging strand, continuous replication, non-continuous replication, and Okazaki fragment)
Vocabulary: DNA Replication
Vocabulary: DNA Replication A. Helicase B. Primase C. Single Strand Binding Protein (SSB) D. Topoisomerase E. Origin of Replication F. DNA Polymerase G. Leading Strand H. Lagging strand I. DNA Ligase J. Okazaki Fragment K. Replication Fork L. RNA Primer M. Topoisomerase .1. Site where the replication of a DNA molecule begins. 2. The new continuous complementary DNA strand synthesized in the direction for the replication fork. 3. A discontinuously synthesized DNA strand that elongates in a direction away from the replication fork 4. Relaxes...
Where would you find histones? A. Part of the RNA polymerase complex that transcribes DNA. B....
Where would you find histones? A. Part of the RNA polymerase complex that transcribes DNA. B. In a virus, helping to "inject" foreign DNA into a cell. C. Bound to an enhancer to regulate transcription. D. Asscoiated with DNA to make chromatin. What is the role of DNA ligase during DNA replication? A. It unwinds the parental double helix. B. It synthesizes RNA nucleotides to make a primer. C. It joins lagging strand (Okazaki) fragments together. D. It stabilizes the...
Which enzyme lays down the signal for DNA polymerase to bind and start creating the complementary...
Which enzyme lays down the signal for DNA polymerase to bind and start creating the complementary strand of DNA? Which enzyme helps relieve tension in the DNA strand as it is pulled apart for replication? What specific structure is needed in order for DNA polymerase to start DNA replication? Select one: a. RNA primer b. DNA primer c. DNA polymerase d. nucleic acid e. nucleotide sequence Which of these is unique to the lagging strand of DNA? Select one: a....
DNA replication involves the enzyme to unwind the double stranded DNA and enzyme to seal the...
DNA replication involves the enzyme to unwind the double stranded DNA and enzyme to seal the Okazaki fragments O Polymerase, ligase O Helicase, polymerase Helicase, ligase Ligase, primase Reset Selection Mark for Review What's This?
Match the following (Total 24 pts) Enzyme responsible for joining DNA strands together The nascent DNA...
Match the following (Total 24 pts) Enzyme responsible for joining DNA strands together The nascent DNA strand that is being synthesized in the same direction as the replication fork. Enz 39 DNA polymerase 40 DNA helicase responsible for transcribing RNA 41DNA sliding clamp 42 Single Stranded Binding Proteins DShort, newly synthesized DNA fragments 43 RNA primer 44 DNA ligase formed on the lagging template strand E Abundle or proteins that assist in the rate of transcription of DNA to mRNA....
Matching the normal function to the enzyme. This enzyme connects Okazaki fragments by forming bonds in the A. DNA backbone. Topoisomerase This enzyme opens the DNA helix, separating the strands to expose B. the bases. Primase This enzyme relaxes supercoils in the DNA that form ahead of the replication fork Helicase DNA Polymerase III This enzyme removes primers by cutting out one RNA nucleotide at a D. time and replacing it with DNA. DNA Polymerase This enzyme uses its own...
unwinds the dna double helix, synthesizes a short complementary
piece of RNA primer, adds complementary nucleotides in 5' to 3'
direction, forms covalent bonds between Okazaki
fragments
Primase [Choose ] DNA polymerase [Choose] DNA ligase [Choose] Helicase [Choose ]
DNA replication involves the enzyme to unwind the double stranded DNA and enzyme to seal the Okazaki fragments O Polymerase, ligase O Helicase, polymerase Helicase, ligase Ligase, primase Reset Selection Mark for Review What's This?
Match the following (Total 24 pts) Enzyme responsible for joining DNA strands together The nascent DNA strand that is being synthesized in the same direction as the replication fork. Enz 39 DNA polymerase 40 DNA helicase responsible for transcribing RNA 41DNA sliding clamp 42 Single Stranded Binding Proteins DShort, newly synthesized DNA fragments 43 RNA primer 44 DNA ligase formed on the lagging template strand E Abundle or proteins that assist in the rate of transcription of DNA to mRNA....
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