Match the component of DNA replication with its best description.
1- Beta clamp ................
2- primer..................
3- Helicase................
4- Topoisomerase...................
5- DNA polymerase I......................
6- DNA polymerase III......................
7- primase......................
8- Ligase......................
9- SSBP......................
10- Telomerase......................
11- Okazaki fragment ......................
Description:
- Replaces RNA
- Break and rejoin covalent bonds
- Provides a 3' OH for DNA polymerase III
- Increases polymerase processivity
- Is an RNA polymerase
- Synthesizes leading strand and Okazaki fragments
- Provides a 5' OH for DNA polymerase I
- Provides a 3' OH for DNA polymerase I
- Completes replication of linear chromosomes
- Provides a 5' OH for DNA polymerase III
- Prevent duplex from re-forming
- Disrupt intermolecular forces
- Creates covalent bond at 5' end of Okazaki fragment
Match the component of DNA replication with its best description. 1- Beta clamp ................ 2- primer.....................
5 Describe the process of DNA replication; include the following terms: antiparallel structure, DNA Okazaki fragments, DNA ligase, primer, primase, helicase, topoisomerase, single-strand binding proteins. Describe the function of Helicase, Primase, topoisomerase, DNA Polymerase III, DNA Polymerase I, DNA ligase
DNA Replication - Describe the process of DNA replication in a well-organized manner. Start with the helicase and go through the entire process, step by step. Use these terms correctly: Helicase, DNA primase, single stranded binding proteins, DNA polymerase I, DNA Polymerase III, leading strand, lagging strand, okazaki fragments, ligase, topoisomerase, sliding clamp, clamp loader. Please use correct descriptions of the enzyme function. For example, do not say, “seals nicks.” Tell me what “seals nicks” means.
unwinds the dna double helix, synthesizes a short complementary
piece of RNA primer, adds complementary nucleotides in 5' to 3'
direction, forms covalent bonds between Okazaki
fragments
Primase [Choose ] DNA polymerase [Choose] DNA ligase [Choose] Helicase [Choose ]
D Question 1 (Matching) Match the enzyme/structure with its role in DNA replication. DNA polymerase synthesizes the new strand of RNA primase Choose synthesizes the new strand of DNA synthesizes a short fragment of complementary RNA attach to template DNA strands to prevent hydrogen bonding Uncoils the supercoil of prokaryotic chromosomes connects Okazaki fragments of new lagging strand of DNA unwinds and unzips the double stranded DNA Single stranded binding proteins (SSB's) DNA Ligase [Choose] Helicase [Choose) DNA Gyrase (Choose]...
In the following diagram, label the following: leading and lagging strand, Okazaki fragment, DNA polymerase, DNA ligase, helicase, RNA primase, singlestrand binding proteins, RNA primer, replication fork, topoisomerase and the 5' and 3' ends of strands.
3. Specifically describe what would occur in DNA replication if the following components were missing, be thoughtful and do not simply state that DNA replication would not occur. Differentiate between prokaryotic and eukaryotic replication when appropriate. DNA ligase- DNA polymerase- Primer- Primase- Topoisomerase- Helicase- SSBP- Sliding clamp/PCNA- Telomerase-
Vocabulary: DNA Replication A. Helicase B. Primase C. Single Strand Binding Protein (SSB) D. Topoisomerase E. Origin of Replication F. DNA Polymerase G. Leading Strand H. Lagging strand I. DNA Ligase J. Okazaki Fragment K. Replication Fork L. RNA Primer M. Topoisomerase .1. Site where the replication of a DNA molecule begins. 2. The new continuous complementary DNA strand synthesized in the direction for the replication fork. 3. A discontinuously synthesized DNA strand that elongates in a direction away from the replication fork 4. Relaxes...
Question 10 A human chromosome has 3 origins of replication. Assuming that all 3 origins of replication are used at the same time, what is the maximum number of helicases present on DNA during DNA replication (not including DNA repair)? 12 O 3 O2 Question 11 Which of the following correctly compares primase and telomerase? O Both primase and telomerase synthesize DNA using a DNA template. O Primase synthesizes RNA using an RNA template, but telomerase synthesizes DNA using a...
QUESTION 10 Below is a list of proteins required for DNA replication to occur and functions involved in DNA replication. Match each protein to its function. helicase A catalyzes phosphodiester bonds between two DNA fragments in a strand single strand binding proteins (SSBPs) Rremoves RNA primer and replaces it with DNA topoisomerase cholds DNA polymerase in place during strand elongation - primase D. breaks hydrogen bonds between base pairs and opens the double helix DNA polymerase in E. extends a...
Match the enzyme activity in DNA synthesis with its function. DNA pol III (_) fragments Helicase (_) Primase (_) using template strand DNA pol I (_) double helix DNA ligase (_) adds nucleotides Topoisomerase (_) primer A. anneals DNA B. relieves overwinding C. adds nucleotides D. unwinds parental E. removes RNA primer, F. adds a short RNA 10 Proofreading and repair of the DNA double helix does NOT involves a. detecting a mis-match in base pairs. b. removing the mis-match...