The process of DNA replication involves four steps,
Initiation: This results in the formation of replication fork. The double stranded DNA are unwind into single stranded structure by the action of the enzyme DNA helicase. One of the DNA strand is oriented in the 3' - 5' direction also known as leading strand and the other is oriented 5' - 3' also known as lagging strand.
Primer binding: a short piece of RNA strand binds to the 3' end known as primer. This process takes place with the help of enzyme DNA primase
Elongation: The enzyme DNA polymerase play an important role in DNA elongation. DNA polymerase 3 binds at the site of primer and adds complimentary base pairs. The synthesis of lagging strands rake place by means of okazaki fragments.
Termination: DNA exonucleases are involved in termination. DNA ligase joins together the okazaki fragments.
5 Describe the process of DNA replication; include the following terms: antiparallel structure, DNA Okazaki fragments,...
DNA Replication - Describe the process of DNA replication in a well-organized manner. Start with the helicase and go through the entire process, step by step. Use these terms correctly: Helicase, DNA primase, single stranded binding proteins, DNA polymerase I, DNA Polymerase III, leading strand, lagging strand, okazaki fragments, ligase, topoisomerase, sliding clamp, clamp loader. Please use correct descriptions of the enzyme function. For example, do not say, “seals nicks.” Tell me what “seals nicks” means.
Describe the process of DNA replication in a well-organized manner. Start with the helicase and go through the entire process, step by step. Use these terms correctly: Helicase, DNA primase, single stranded binding proteins, DNA polymerase I, DNA Polymerase III, leading strand, lagging strand, okazaki fragments, ligase, topoisomerase, sliding clamp, clamp loader. Please use correct descriptions of the enzyme function. For example, do not say, “seals nicks.” Tell me what “seals nicks” means. If you speak about something you have...
Write a concept map with the following terms. DNA Complementarity of bases Antiparallel Semiconservative replication Origin of replication Replication fork Helicase SSBP Topoisomerase RNA Primase Polymerization proceeds 5’ to 3’ DNA polymerase Leading strand Lagging strand Okasaki fragments DNA ligase Bidirectional replication Telomeres Telomerase PCR Gel electrophoresis Restriction enzymes Palindromic sequence Southern blots Sanger dideoxy sequencing Genetic engineering Recombinant DNA Transgenic Organism GMOs
Describe the process of DNA replication. (Your answer should include the following: replication fork, semiconservative replication, replication fork, DNA gyrase, helicase, primase, DNA polymerase, DNA ligase, leading strand, lagging strand, continuous replication, non-continuous replication, and Okazaki fragment)
1. During DNA replication the lagging strand is complemented with Okazaki fragments that still contain the RNA primer. Which pair of enzymes removes the RNA primer and seals the DNA nicks? DNA polymerase I and ligase the DNA polymerases II and III primase and gyrase DNA polymerase III and helicase DNA polymerase III and primase 2. Which of the following best describes the process of DNA replication in a prokaryote? Replication begins at multiple sites, spreading outward until the entire...
In the following diagram, label the following: leading and lagging strand, Okazaki fragment, DNA polymerase, DNA ligase, helicase, RNA primase, singlestrand binding proteins, RNA primer, replication fork, topoisomerase and the 5' and 3' ends of strands.
Vocabulary: DNA Replication A. Helicase B. Primase C. Single Strand Binding Protein (SSB) D. Topoisomerase E. Origin of Replication F. DNA Polymerase G. Leading Strand H. Lagging strand I. DNA Ligase J. Okazaki Fragment K. Replication Fork L. RNA Primer M. Topoisomerase .1. Site where the replication of a DNA molecule begins. 2. The new continuous complementary DNA strand synthesized in the direction for the replication fork. 3. A discontinuously synthesized DNA strand that elongates in a direction away from the replication fork 4. Relaxes...
Match the component of DNA replication with its best description. 1- Beta clamp ................ 2- primer.................. 3- Helicase................ 4- Topoisomerase................... 5- DNA polymerase I...................... 6- DNA polymerase III...................... 7- primase...................... 8- Ligase...................... 9- SSBP...................... 10- Telomerase...................... 11- Okazaki fragment ...................... Description: - Replaces RNA - Break and rejoin covalent bonds - Provides a 3' OH for DNA polymerase III - Increases polymerase processivity - Is an RNA polymerase - Synthesizes leading strand and Okazaki fragments ...
QUESTION 10 Below is a list of proteins required for DNA replication to occur and functions involved in DNA replication. Match each protein to its function. helicase A catalyzes phosphodiester bonds between two DNA fragments in a strand single strand binding proteins (SSBPs) Rremoves RNA primer and replaces it with DNA topoisomerase cholds DNA polymerase in place during strand elongation - primase D. breaks hydrogen bonds between base pairs and opens the double helix DNA polymerase in E. extends a...
D Question 1 (Matching) Match the enzyme/structure with its role in DNA replication. DNA polymerase synthesizes the new strand of RNA primase Choose synthesizes the new strand of DNA synthesizes a short fragment of complementary RNA attach to template DNA strands to prevent hydrogen bonding Uncoils the supercoil of prokaryotic chromosomes connects Okazaki fragments of new lagging strand of DNA unwinds and unzips the double stranded DNA Single stranded binding proteins (SSB's) DNA Ligase [Choose] Helicase [Choose) DNA Gyrase (Choose]...