Question

In rho-dependent transcription termination: the formation of a hairpin in the transcribed mRNA causes RNA polymerase to pause, facilitating termination. rho binds the mRNA, and when it makes contact with RNA polymerase, it assists with the removal of the mRNA from the DNA template. the rho factor binds to the -10 consensus sequence located in the promoter region to terminate transcription. a site within the poly(A) tail is cleaved which signals termination. the 3' untranslated region (3" UTR) is synthesized.

Answer 1

1. Answer- rho binds the mRNA, when it makes contact with DNA polymerase, it assists with the removal of mRNA from DNA template.

Explanation- In case of rho dependent termination a protein named Rho is required for termination activity. This protein is an ATP-dependent and RNA stimulated helicase that disrupts the the nascent DNA- RNA hybrids. It translocates along RNA in 5' to 3' direction until it reaches the RNA-DNA hybrid in RNA polymerase. Where it releases RNA from DNA.

2. Answer- DNA Primase.

Explanation-

DNA polymerase did not have capability to autonomously synthesise DNA without having three prime hydroxyl group.

DNA Primase is actually a RNA polymerase which which synthesizes a short stretch of RNA using DNA template and provides a free 3 prime hydroxyl group for further the synthesis of DNA.

3. Answer- the 500 bp fragment

Explanation- During gel electrophoresis mostly smaller fragments moves at a faster rate as compared to larger fragments. Therefore 500bp fragment will move farthest.