Question

The subject is Biochemistry but I see that os not a subject to choose on here. Out of All five sheets what ever questions you can answer would be great I need them to study for a test I have tomorrow

Lecture 4 Calculate charge of a peptide at extremely high and low pH given the amino acid sequence. I will furnish you with a formula sheet with a side-chain pKa table. What is the exome? Why is alternative splicing important? What's the difference between a protein and a peptide? What is a peptide bond? What atoms are involved in a peptide bond? What are primary, secondary, tertiary, and quaternary structure? Be able to identify alpha helices, beta sheets, loops/turns

Answer 1

1) Exome is the part of genome having only exons and not introns. This exome is responsible for protein amino acid acid sequence and coded for protein.

2) Alternative splicing is important as because of this many product proteins are formed from a single mRNA , thus having alternate products. This results in increased no. Of and diverse protein products from less number of genes.

3) A protein is a product of joining together and getting final quaternary structure to the 2 or more polypeptide chains and forming an enzyme or any other protein.

Whereas peptides are short chains of amino acids joined together with help of peptide bonds

4) A bond between 2 amino acids formed by removal of a water molecule.

This bond is formed between NH4+ group of ok ne amino acid and -COOH group of other.

This forming CO-NH peptide bond by removal of water.

5)primary structure - the sequence of amino acids

Secondary- the folding of polypeptide chains in alpha helix or beta pleated.

Tertiary - is arrangement of these chains and further folding in globose or other shapes.

Quaternary- coming together and bonding of tertiary structures of 2-3 peptide chains. Thus , finally forming a protein and giving final shape.