Understand how non homologous end joining proteins and homology-directed repair work
Understand how non homologous end joining proteins and homology-directed repair work, how they are used in DNA repair, and how they are used in CRISPR/Cas9. Also understand the basics of how CRISPR/Cas9 can be used to alter DNA sequences.
Homework Answers
Non homologous end joining and homology directed DNA repair frequency of double stranded breaks introduced by genome editing reagents.
Genome editing using transcription activator like effector nucleases allows one to precisely engineer desired changes within a given target sequence.
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Understand how non homologous end joining proteins and homology-directed repair work
Question 5 1 pts Non-homologous end joining is "error prone" because There is no template used...
Question 5 1 pts Non-homologous end joining is "error prone" because There is no template used during repair The DNA polymerase invovled adds incorrect bases ODNA ends are often trimmed before repair Damaged bases are incorrectly paired during repair Question 6 1 pts Global genomic and transcription coupled are the two sub-types of nucleotide excision repair. Select true statements relevant to these processes Both can detect damage involving multiple damaged bases Both involve proteins that recognize the damaged bases Both...
4. The CRISPR-Cas9 system is an important new technique in molecular biology. What is the natural...
4. The CRISPR-Cas9 system is an important new technique in
molecular biology. What is the natural function of this system?
Describe how you would use this system to generate a null mutation
in another organism (i.e. explain Figure 6-43). How does it work?
What is the modification of the method that allows for correction
of a mutation (e.g. the mouse crystalline gene)? And lastly, what
are the problems with the CRISPR system?
FIGURE 6-43 Single-nucleotide mutations
can be introduced into...
Describe how Nucleotide Excision Repair, Homologous Recombination Repair (HRR)
Describe how Nucleotide Excision Repair, Homologous Recombination Repair (HRR), and Non-homologous End Joining (NHEJ) are beneficial for repair of damaged DNA. If you could choose between having HRR and NHEJ performed, which would you choose and why?
Which of the following statements is FALSE about double-strand breaks (DSBs)? A. Non-homologous end-joining between the...
Which of the following statements is FALSE about double-strand breaks (DSBs)? A. Non-homologous end-joining between the ends of two DSBs can create inversions or translocations. B. Spo11-mediated DSBs initiate meiotic recombination. C. Unrepaired DSBs are lethal to a cell. D. Unrepaired DSBs trigger a checkpoint that arrests cells in G2. E. Cas9 can be targeted to make a DSB at a specific genomic sequence by annealing a small piece of single-stranded DNA that is complementary to the cut site.
Please help me answering these multiple questions of how the CRISPR system works, thank you! 1....
Please help me answering these multiple questions of how the CRISPR system works, thank you! 1. Match the CRISPR component with its function. (matching the upper case letters to the lower case letters) A). Cas9 B). gRNA (sometimes called sgRNA) C). Non-homologous End Joining (NHEJ) D). Homology-directed Repair (HDR) a). A short RNA molecule that guides a DNA cutting enzyme to a specific location in genomic DNA. b). A type of DNA repair mediated by a set of enzymes all...
Haploid yeast cells that preferentially repair double-strand breaks by homologous recombination (rather than by non-homologous end...
Haploid yeast cells that preferentially repair double-strand breaks by homologous recombination (rather than by non-homologous end joining) are especially sensitive to agents that cause double-strand breaks in DNA. If the breaks occur in the G1 phase of the cell cycle, most cells will die; however, if the breaks occur in the G2 phase, a much higher fraction of cells survive. Why do you suppose this is?
1) When DNA is damaged, a cell has a variety of repair pathways available to correct...
1) When DNA is damaged, a cell has a variety of repair pathways available to correct the problem. Discuss their timing, anything they are good at detecting, and explain their specific mechanisms. A) 3pts - Mismatch repair: B) 3pts - Base excision [BER] repair: C) 3pts - Strand breakage repair – compare HRR [homologous recombination repair] and NREJ [non-recombination end joining] 2) 5pts - Discuss how an E. coli origin of replication is recognized [sequences?], opened [by dnaA], and...
Homologous Recombination A. How are the repair of double strand breaks (DSBs) and production of DSBs...
Homologous Recombination A. How are the repair of double strand breaks (DSBs) and production of DSBs for recombination connected? B. How does the general model of strand invasion, branch migration, and resolution of the Holliday junction work? C. How do RecA and RecBCD function to promote recombination? D. Endonuclease activity of RecBCD is greater in the 3'-5' strand, rather than the 5'-3' strand. Describe how this affects strand invasion, branch migration and resolution. E. How is recombination initiated during meiosis...
1. Homologous recombination can happen between non-identical DNA sequences. T/F? 2. Homologous recombination can happen in_______...
1. Homologous recombination can happen between non-identical DNA sequences. T/F? 2. Homologous recombination can happen in_______ a) meiosis b) mitosis c) both 3. Homologous recombination in meiosis has the main purpose of_____ a) DNA repair b) Creating new chromosomes c) Sealing double-stranded breaks 4. Strand invasion usually happens without enzymatic assistance. T/F? 5. When replication fork runs into a nick, it results in a_______ a) single-stranded break b) double-stranded break 6. The invading end is usually a _______ a) 3'...
Mismatch repair requires all of the following EXCEPT: Requires information from a nearby homologous chromosome Uses...
Mismatch repair requires all of the following EXCEPT: Requires information from a nearby homologous chromosome Uses information on the parental strand to identify the error. Is tightly associated with new replicated DNA Makes use of ligase. Requires the use of a repair DNA polymerase. Question 12 Which of the following is NOT necessary for homologous recombination to occur? O nucleases 5' DNA strand overhangs 3' DNA strand overhangs ligase a long stretch of sequence similarity Which of the following apply...
Question 5 1 pts Non-homologous end joining is "error prone" because There is no template used during repair The DNA polymerase invovled adds incorrect bases ODNA ends are often trimmed before repair Damaged bases are incorrectly paired during repair Question 6 1 pts Global genomic and transcription coupled are the two sub-types of nucleotide excision repair. Select true statements relevant to these processes Both can detect damage involving multiple damaged bases Both involve proteins that recognize the damaged bases Both...
4. The CRISPR-Cas9 system is an important new technique in
molecular biology. What is the natural function of this system?
Describe how you would use this system to generate a null mutation
in another organism (i.e. explain Figure 6-43). How does it work?
What is the modification of the method that allows for correction
of a mutation (e.g. the mouse crystalline gene)? And lastly, what
are the problems with the CRISPR system?
FIGURE 6-43 Single-nucleotide mutations
can be introduced into...
Haploid yeast cells that preferentially repair double-strand breaks by homologous recombination (rather than by non-homologous end joining) are especially sensitive to agents that cause double-strand breaks in DNA. If the breaks occur in the G1 phase of the cell cycle, most cells will die; however, if the breaks occur in the G2 phase, a much higher fraction of cells survive. Why do you suppose this is?
Mismatch repair requires all of the following EXCEPT: Requires information from a nearby homologous chromosome Uses information on the parental strand to identify the error. Is tightly associated with new replicated DNA Makes use of ligase. Requires the use of a repair DNA polymerase. Question 12 Which of the following is NOT necessary for homologous recombination to occur? O nucleases 5' DNA strand overhangs 3' DNA strand overhangs ligase a long stretch of sequence similarity Which of the following apply...
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