What does the GATC methylation site do?
In E. coli downstream GATC sequences are methylated, promoting transcription. For example, pyelonephritis-associated pili (PAP) phase variation in uropathogenic E. coli is controlled by Dam through the methylation of the two GATC sites proximal and distal to the PAP promoter.
Methylation of GATC sites in the dnaAp2 promoter, two of which are in the -35 and -10 sequences, has been predicted to play an important role in regulating dnaA gene expression during the cell cycle because the promoter is sequestered from methylation immediately following replication.
What would be the expected outcome of a mutation in E.coli that prevented methylation of adenine in the sequence 5'-GATC-3'? A) loss of epigenetic markers B) Randomization of mismatch repair C) increased mutation rate D) B and C E) All of these
QUESTION 5 In what way does DNA methylation typically regulate transcription? A. Represses transcription. B. Activates transcription. O C. It doesn't regulate transcription at all. D. Both A and B.
Normally the FMR1 locus does not have DNA methylation in the gene or promoter region. However, copies of the FMR1 gene with mutation length CGG repeats are modified; they have DNA methylation extending into the promoter region. This leads to ____________ expression of that copy of the FMR1 gene. no change in decreased increased
What is DNA methylation? Describe how this mechanism regulates the expression of genes. What can happen when the DNA is hyper-methylated? Pick and describe a disease that results from DNA hyper-methylation.
What is phishing, and how does it work? Spotting a fake site is not as easy as you might think. How about viruses, spyware, and adware? Besides keeping your antivirus software up to date, what are some other steps you can take to either prevent virus infection or minimize the effects if you do get infected?
Which does not affect gene expression at the post-transcriptional level? Choose one: O A. DNA methylation O B. small interfering RNAs O C. microRNAs O D. nucleotide sequences in the untranslated regions of an mRNA
Transcriptional Regulation Gene activation/deactivation 1. what is histone acetylation? How does it affect gene expression? What else can happen to nucleosomes that affects gene expression? Do all the nucleosomes fall off the chromosome at once? 2. what is DNA methylation? How does it affect gene expression? Be able to discuss the examples of DNA methylation covered in class (X inactivation, imprinting). 3. Epigenetics. What is this, and how does it affect gene expression?
In what way is H3K27me3-dependent imprinting different from DNA methylation and what are the implications of this difference?
What are the roles of DNA methylation and epigenetic reprogramming of paternal/ maternal and embryonic genomes ?
How does isoleucine 16 stabalize chymotrypsin active site? what would raising the pH well past 8 do?