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What would be the expected outcome of a mutation in E.coli that prevented methylation of adenine...

What would be the expected outcome of a mutation in E.coli that prevented methylation of adenine in the sequence 5'-GATC-3'?

A) loss of epigenetic markers

B) Randomization of mismatch repair

C) increased mutation rate

D) B and C

E) All of these

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Answer #1

Option E, all of these

GATC , adenine is methylated this act as epigenetic marker to mark parental stand , and newly synthesized DNA is not methylated for short duration.

In this hemimethylated state, demethylated DNA is sense as new DNA stand and subjected to mismatch repair . By methylation of A in GATC , parental strand in protected from mismatch repair. If methylation do not occur then marking of new DNA stand will not be possible and lead to randomization of mismatch repair.

Due to randomization of repair mechanism, there will be increased rate of mutation can occur.

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