Homework Answers
Answer-
Strain l has tetracyclin resistance gene in its chromosome and vancomycin resistance gene in plasmid so that this strain l is resistance to tetracyclin and vancomycin. So that strain l can grow on tetracyclin and/or vancomycin containing media but not on ampicillin containing media.
Strain ll has ampicillin resistance gene in its chromosome so that strain ll is resistance to ampicillin. Strain ll can grow on ampicillin containing media but not on tetracyclin and/or vancomycin containing media.
| Strain l | Strain ll | |
| Plain agar | + | + |
| Agar+Amp | - | + |
| Agar+Tet | + | - |
| Agar+Van | + | - |
| Agar+Van+Tet | + | - |
| Agar+Van+Amp | - | - |
| Agar+Tet+Amp | - | - |
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Name Jumping Genes Assignment #1 Strain 1 Strain II Tet tetracycline Van - vancomycin Amp =...
Strain ! Strain II Amper Pen = penicillinC = chromosome Kan = kanyomycin P = plasmid...
Strain ! Strain II Amper Pen = penicillinC = chromosome Kan = kanyomycin P = plasmid Amp - ampicillinr = resistance Tet tetracycline Strains I and II are plated on "confirmation" plates, Fill in the table with a + or - according to whether ye think the bacteria will grown on the plate (+) or not (-). Strain ! Strain II + T Fronte + Amp + Tet + Kan + Pen + Kan + Tet + Kan + Amp...
.2 . . .3 Agar + Kan Agar + Pen Agar + Kan + Tet Agar...
.2 . . .3 Agar + Kan Agar + Pen Agar + Kan + Tet Agar + Kan + Amp Agar +Tet + Amp Agar + Pen +Kan Agar +Tet + Pen 2. Strains I and II undergo conjugation and colonies from the "mating plate are put on the following agar plates with antibiotics. Look at the results in the following table: 1 Strain III + E S Plain agar38832 Agar + Ampe SENS Agar +Tet Agar+Kan Agar-Pen 03 Agar...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
Protein P is synthesized in relatively high amounts in the human pancreas. This protein has been...
Protein P is synthesized in relatively high amounts in the human pancreas. This protein has been isolated and purified, but its amino acid sequence has not been determined. We wish to clone the gene for protein P. (a) How can a probe be prepared to identify the gene for protein P? (b) If we have prepared a radioactive messenger RNA as our probe in part (a), how could we verify that it is the mRNA for protein P? (c) If...
Strain ! Strain II Amper Pen = penicillinC = chromosome Kan = kanyomycin P = plasmid Amp - ampicillinr = resistance Tet tetracycline Strains I and II are plated on "confirmation" plates, Fill in the table with a + or - according to whether ye think the bacteria will grown on the plate (+) or not (-). Strain ! Strain II + T Fronte + Amp + Tet + Kan + Pen + Kan + Tet + Kan + Amp...
.2 . . .3 Agar + Kan Agar + Pen Agar + Kan + Tet Agar + Kan + Amp Agar +Tet + Amp Agar + Pen +Kan Agar +Tet + Pen 2. Strains I and II undergo conjugation and colonies from the "mating plate are put on the following agar plates with antibiotics. Look at the results in the following table: 1 Strain III + E S Plain agar38832 Agar + Ampe SENS Agar +Tet Agar+Kan Agar-Pen 03 Agar...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
Protein P is synthesized in relatively high amounts in the human pancreas. This protein has been isolated and purified, but its amino acid sequence has not been determined. We wish to clone the gene for protein P. (a) How can a probe be prepared to identify the gene for protein P? (b) If we have prepared a radioactive messenger RNA as our probe in part (a), how could we verify that it is the mRNA for protein P? (c) If...
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