Serial dilutions question: You begin with an original solution of a drug named 2-APB, which is...
Serial dilutions question:
You begin with an original solution of a drug named 2-APB, which is dissolved in DMSO and has a concentration of 50mg/mL. How would you use serial dilutions to make 25µL of final solution of 2-APB in DMSO that has a concentration of 20ng/µL?
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Serial dilutions question:
You begin with an original solution of a drug named 2-APB, which
is...
Serial Dilution Questions 1, Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution...
Serial Dilution Questions
1, Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 350.0 mg/L and do the following set of serial dilutions on it: 5.00 mL of stock diluted to a total volume (TV) of 500.00 mL (Dilution 1); 2,00 mL of Dilution 1 is then diluted to a TV of 250.00 mL (Dilution 2); finally, 1.00 mL of Dilution 2 is then diluted to a total volume of...
You carry out a serial dilution of a bacterial culture, using dilutions of ⅕, ⅕, 1/100,...
You carry out a serial dilution of a bacterial culture, using dilutions of ⅕, ⅕, 1/100, and 1/10. Then you plate 100 µl of the final dilution and the next day observe 200 colonies on your plate. What was the concentration of the original culture? (5 pts)
1. Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 3...
1. Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 350.0 mg/L and do the following set of serial dilutions on it: 5.00 mL of stock diluted to a total volume (TV) of 500.00 mL (Dilution 1); 2.00 mL of Dilution 1 is then diluted to a TV of 250.00 mL (Dilution 2); finally, 1.00 mL of Dilution 2 is then diluted to a total volume of 100.00 mL. Calculate...
In the lab, you will need to perform serial dilutions. What would be the concentration of...
In the lab, you will need to perform serial dilutions. What would be the concentration of the new solution made if you took 2.50 mL of a 0.10 M Cu(2+) solution and diluted it to 10.00 mL?
You start with a 4M stock solution of NaCl, you need to make dilutions to obtain...
You start with a 4M stock solution of NaCl, you need to make dilutions to obtain various working solutions. Describe how you would make 100 mL of a working solution that has a final concentration of 1M. Be sure to include the volume and concentration of the components.
2) You are given a bacterial culture with the following serial dilutions: 1 ml 0.1 ml...
2) You are given a bacterial culture with the following serial dilutions: 1 ml 0.1 ml 1 ml Original culture 9 ml media 9.9 ml media 9 ml media B a) what is the final dilution in tube C? (1 pt) b) if you plated 100 ul of the bacterial dilution in tube B, incubated and counted 114 colonies the next day, what would the CFU/ml be of the original bacterial culture? (show work and units- 2 pts)
You prepared a set of standard solutions by serial dilutions. You started with a stock solution...
You prepared a set of standard solutions by serial dilutions. You started with a stock solution of bromocresol green with a concentration of 0.213 M. The following standard solutions were prepared as described below. Solution A: Stock solution of bromocresol green at a concentration of 0.213 M. Solution B: 25 mL of solution A was diluted to 50 mL in a volumetric flask. Solution C: 25 mL of solution B was diluted to 50 mL in a volumetric flask. Solution...
You prepared four standard solutions by serial dilutions that contained decreasing concentrations of bromocresol green, but...
You prepared four standard solutions by serial dilutions that contained decreasing concentrations of bromocresol green, but constant HCl concentration. You also prepared four additional standard solutions by serial dilutions that contained decreasing concentrations of bromocresol green, but constant NaOH concentration. You then measured the absorbances of these solutions at their respective lamba max wavelengths (AX1 & A2). These absorbances were plotted versus Hin and In molar concentrations respecti vely to produce linear calibration curves with linear equations shown below. AX1...
b. 1 ml 1ml 「-I Final dilution 4(1 point) This represents a serial dilution to determine...
b. 1 ml 1ml 「-I Final dilution 4(1 point) This represents a serial dilution to determine viable bacterial counts. Indicate the final dilutions in each of the two tubes. Note that two different volumes of the cells intube #2 are plated onto agar plates A and B. Indicate the dilution factor that will be used to report the original cell concentration on a per mL basis. 1 ml 1 ml Final dilution in Tube #1 = Final dilution in Tube...
You only have 150 pl stock solution that contains 100 mgml of protein. You want to...
You only have 150 pl stock solution that contains 100 mgml of protein. You want to make a set of standards with the concentrations shown below. You need a minimum of 500 l of each standard left after you have finished all your dilutions. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below include the final dilution of the standard as compared to the original stock concentration and the final...
Serial Dilution Questions
1, Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 350.0 mg/L and do the following set of serial dilutions on it: 5.00 mL of stock diluted to a total volume (TV) of 500.00 mL (Dilution 1); 2,00 mL of Dilution 1 is then diluted to a TV of 250.00 mL (Dilution 2); finally, 1.00 mL of Dilution 2 is then diluted to a total volume of...
1. Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 350.0 mg/L and do the following set of serial dilutions on it: 5.00 mL of stock diluted to a total volume (TV) of 500.00 mL (Dilution 1); 2.00 mL of Dilution 1 is then diluted to a TV of 250.00 mL (Dilution 2); finally, 1.00 mL of Dilution 2 is then diluted to a total volume of 100.00 mL. Calculate...
2) You are given a bacterial culture with the following serial dilutions: 1 ml 0.1 ml 1 ml Original culture 9 ml media 9.9 ml media 9 ml media B a) what is the final dilution in tube C? (1 pt) b) if you plated 100 ul of the bacterial dilution in tube B, incubated and counted 114 colonies the next day, what would the CFU/ml be of the original bacterial culture? (show work and units- 2 pts)
You prepared a set of standard solutions by serial dilutions. You started with a stock solution of bromocresol green with a concentration of 0.213 M. The following standard solutions were prepared as described below. Solution A: Stock solution of bromocresol green at a concentration of 0.213 M. Solution B: 25 mL of solution A was diluted to 50 mL in a volumetric flask. Solution C: 25 mL of solution B was diluted to 50 mL in a volumetric flask. Solution...
You prepared four standard solutions by serial dilutions that contained decreasing concentrations of bromocresol green, but constant HCl concentration. You also prepared four additional standard solutions by serial dilutions that contained decreasing concentrations of bromocresol green, but constant NaOH concentration. You then measured the absorbances of these solutions at their respective lamba max wavelengths (AX1 & A2). These absorbances were plotted versus Hin and In molar concentrations respecti vely to produce linear calibration curves with linear equations shown below. AX1...
b. 1 ml 1ml 「-I Final dilution 4(1 point) This represents a serial dilution to determine viable bacterial counts. Indicate the final dilutions in each of the two tubes. Note that two different volumes of the cells intube #2 are plated onto agar plates A and B. Indicate the dilution factor that will be used to report the original cell concentration on a per mL basis. 1 ml 1 ml Final dilution in Tube #1 = Final dilution in Tube...
You only have 150 pl stock solution that contains 100 mgml of protein. You want to make a set of standards with the concentrations shown below. You need a minimum of 500 l of each standard left after you have finished all your dilutions. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below include the final dilution of the standard as compared to the original stock concentration and the final...
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