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2) You are given a bacterial culture with the following serial dilutions: 1 ml 0.1 ml...
Dilution Practice Problem #5 You are given the following bacterial dilution scheme: ml 1 ml 1...
Dilution Practice Problem #5 You are given the following bacterial dilution scheme: ml 1 ml 1 ml 1 ml 1 ml CODILE 9ml 9ml 9ml 0.1 ml 0.1 ml colonies) (colonies colonies) Individual tube dilutions: BCD Dilution Factor: Total tube dilutions) IDE AB Plated dilution factor 1. Which plate is the countable plate? (30-300 countable range) 2. Calculate the titer of the original culture in cfu/ml.
Problem#1 ctice en the following bacterial dilution scheme Name: 1 ml 1 ml 1 ml 1 ml Bacterial cu...
Problem#1 ctice en the following bacterial dilution scheme Name: 1 ml 1 ml 1 ml 1 ml Bacterial culture 4 ml 9 ml 9ml 2ml 0.1 m 1 ml1 m0.1 m 400 CFU 400 CFU 140 CFU 16 CFU Individual tube dilutions Dilution Factor (-Total tube dilutions) Plated dilution factor 1. Explain why plates E and F have the same colony count. 2. Which plate is the countable plate? (30-300 countable range) Calculate the titer of the original culture in...
You carry out a serial dilution of a bacterial culture, using dilutions of ⅕, ⅕, 1/100,...
You carry out a serial dilution of a bacterial culture, using dilutions of ⅕, ⅕, 1/100, and 1/10. Then you plate 100 µl of the final dilution and the next day observe 200 colonies on your plate. What was the concentration of the original culture? (5 pts)
please help with whatever possible. thank you so much in advance. Name One use of serial...
please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water....
A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water. (2) 10uL from (1) are then added to 9.99mL of water. (3) A 10-2 dilution is made from tube # (2). (4) 100uL from (3) are plated for a pour plate and incubated. There were 34 colonies counted on one quarter of the plate following incubation. a) What was the overall dilution? b) How many cfu/mL were present in the original culture? c) How many...
17. A sample of a culture of bacteria is subjected to 10-fold serial dilution; 0.1 mL...
17. A sample of a culture of bacteria is subjected to 10-fold serial dilution; 0.1 mL aliquots of the dilutions are grown on agar plates and the number of colonies counted: Dilution: Number of colonies: 10- Too many to count 10-6 10- 2 0 -3 Too many 249 to count 10-4 24 2 10-5 0 BIOT 5021-SP2 Assuming that one cell gives rise to one colony, how many cells were there per mL of the original culture?
Hello, just wanted to check my answers. Answer as many as you like/can. Thankyou. 1) Four...
Hello, just wanted to check my answers. Answer as many as you like/can. Thankyou. 1) Four grams of soil are added to 36 ml sterile water and mixed well. 0.1 ml of this mix was added to 9.9 ml sterile water. This was diluted by 4 successive 1/10 dilutions. 1.0 ml from the last dilution was used to prepare a pour plate. After incubation 289 colonies were counted. Calculate the # CFU/g soil. 2) A 0.1 ml aliquot of a...
data given Procedure for Serial Dilutions: Experimental Data is given II ml 1 ml ml ml...
data given
Procedure for Serial Dilutions: Experimental Data is given II ml 1 ml ml ml I ml Tube 11 Tube 21 Tube 3 Tube 4 Il ml 1 ml ml No of bacterial Colonies TNTC TNTC 290 35 Using the Pour-Plate Method What was the purpose of this lab exercise? Complete the following table with your observations: Dilution factor Comments Number of bacteria per 1 ml of original milk Number of colonies on the plate 10 100 1,000 10,000...
1. Calculate the D.F for a bacterial culture if 2000uL of undiluted cuiture was added to a nutrie...
#1-6
1. Calculate the D.F for a bacterial culture if 2000uL of undiluted cuiture was added to a nutrient broth to bring up the final volume to 1 liter. (2 pts) a. 102 b. 2 X 10 c. 2 X 104 2. It you plated 20 ul of sample with a dilution factor of 10-3, what would your total sample volume be? (Total sample volume - (Volume plated) x (Dilution)). (2 pts) 3. Calculate the O.C.D using a viable count...
2. A series of 1 ml samples from tube #4 of the dilution s eries below are spread on agar plates,...
2. A series of 1 ml samples from tube #4 of the dilution s eries below are spread on agar plates, resulting in the growth of an average of 68 colonies per plate. What is the concentration of colony-forming units (cfu/mi) present in the original culture? 0.1 ml 0.1 ml 1 ml 1 ml 100 ml 9.9 ml 9.9 ml 9 ml 9 ml 2 4 3. The culture flask at left contains a total of 3.2 x 10s cfu....
Dilution Practice Problem #5 You are given the following bacterial dilution scheme: ml 1 ml 1 ml 1 ml 1 ml CODILE 9ml 9ml 9ml 0.1 ml 0.1 ml colonies) (colonies colonies) Individual tube dilutions: BCD Dilution Factor: Total tube dilutions) IDE AB Plated dilution factor 1. Which plate is the countable plate? (30-300 countable range) 2. Calculate the titer of the original culture in cfu/ml.
Problem#1 ctice en the following bacterial dilution scheme Name: 1 ml 1 ml 1 ml 1 ml Bacterial culture 4 ml 9 ml 9ml 2ml 0.1 m 1 ml1 m0.1 m 400 CFU 400 CFU 140 CFU 16 CFU Individual tube dilutions Dilution Factor (-Total tube dilutions) Plated dilution factor 1. Explain why plates E and F have the same colony count. 2. Which plate is the countable plate? (30-300 countable range) Calculate the titer of the original culture in...
please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
17. A sample of a culture of bacteria is subjected to 10-fold serial dilution; 0.1 mL aliquots of the dilutions are grown on agar plates and the number of colonies counted: Dilution: Number of colonies: 10- Too many to count 10-6 10- 2 0 -3 Too many 249 to count 10-4 24 2 10-5 0 BIOT 5021-SP2 Assuming that one cell gives rise to one colony, how many cells were there per mL of the original culture?
data given
Procedure for Serial Dilutions: Experimental Data is given II ml 1 ml ml ml I ml Tube 11 Tube 21 Tube 3 Tube 4 Il ml 1 ml ml No of bacterial Colonies TNTC TNTC 290 35 Using the Pour-Plate Method What was the purpose of this lab exercise? Complete the following table with your observations: Dilution factor Comments Number of bacteria per 1 ml of original milk Number of colonies on the plate 10 100 1,000 10,000...
#1-6
1. Calculate the D.F for a bacterial culture if 2000uL of undiluted cuiture was added to a nutrient broth to bring up the final volume to 1 liter. (2 pts) a. 102 b. 2 X 10 c. 2 X 104 2. It you plated 20 ul of sample with a dilution factor of 10-3, what would your total sample volume be? (Total sample volume - (Volume plated) x (Dilution)). (2 pts) 3. Calculate the O.C.D using a viable count...
2. A series of 1 ml samples from tube #4 of the dilution s eries below are spread on agar plates, resulting in the growth of an average of 68 colonies per plate. What is the concentration of colony-forming units (cfu/mi) present in the original culture? 0.1 ml 0.1 ml 1 ml 1 ml 100 ml 9.9 ml 9.9 ml 9 ml 9 ml 2 4 3. The culture flask at left contains a total of 3.2 x 10s cfu....
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