
Practice Problem 1 A restriction map for a fictitious 4000 base pair plasmid is shown in...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
B4. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with the enzymes BamHI, EcoRI and Ndel. The digests are carried out with each enzyme alone and then with different combinations of the three enzymes. Enzyme Fragment sizes kb BamHI 1.4 10.6 EcoRI 4.5 7.5 Ndel 2.5 9.5 BamHI + EcoRI 45 3.4 1.4 BamHI + Ndel 1.4 2.5 5.9 EcoRI + Ndel 0.5 2.0 2.5 7.0 (a) Draw an agarose gel with restriction fragments...