Question

Name at least three factors that would decrease the titer of a given phage preparation.

Drew, who is incredibly conscientious and cares deeply about the environment, decided it would be best to use the same pair of gloves throughout today’s procedure. Why is this, although good for the environment, a pretty bad ideas for todays procedure?

Answer 1

Question-1:

Answer:

Phage titer: The number of plaque-forming units (PFU/ml) = no of cells/ volume of dilution x volume of diluted sample added to the plate. The lytic and lysogenic phases of given phage preparation completely depend upon teh following factors.

Three factors that would decrease the titer of a given phage preparation:

1. Temperature: If the temperature increases in the medium leading to the decrease in phage titer

2. PH : At optimum PH phage titer value will be good. At lower PH, titer value of an individual phage will be reduced.

3. Nutrient availbility (broth) with differential medium