Question

7. Mix the contents of tube 6 and 7, pour into a cuvette, and record the absorbance measurements. Now plot the values in table 2 on one panel of the graph paper at the end of the exercise. The horizontal axis should be the independent variable and the vertical axis the dependent variable. In this experiment what is the dependent variable? absorbance Plot all three tests on the same coordinates using different plotting symbols. Draw a single straight line that best fits the points for each of the conditions. (Curves may plateau at the end.) In mathematical terms, what characteristic of the graphed line is a measure of enzyme activity? Which amount of enzyme gave a linear absorbance change from 0 to 1 in approximately 120 seconds? Change the amount of enzyme called for in mixing tables 3 & 5 as necessary. The standardizing procedure must be repeated for each batch of extract. Why?

these are the numbers of the graph, i just need the questions answered

Answer 1

Absorbance

In this experiment absorbance is the dependent variable since it changes based on concentration of enzymes

Enzhme activity depends upon absorbance value . Hence change in absorbance or increase in absorbance in the graph is measure of increase in enzyme activity

1 ml extract showed linear line showing absorbance if 0 to 1 in aporoximately 120 seconds

Since enzyme concentration alters the enzyme activity, it will be changed for every batch and it has to standardized