You isolate a piece of DNA from a microorganism you cultivated from your teeth. The piece...
You isolate a piece of DNA from a microorganism you cultivated from your teeth. The piece of DNA is 94 kbp is size and is circular. You sequence it and discover that it contains genes for capsule formation, pili, and antibiotic resistance, as well as an origin of replication. What is this piece of DNA and how is it related to the other genetic elements found in prokaryotic cells?
Homework Answers
This piece of double stranded circular DNA is called a plasmid. It is and extra-chromosomal DNA that's floats around in the cytosol of prokaryotes but distinctly away from the nucleoid region. It mostly possesses genes which provides additional advantages to the organism in the given environment and can be ridden of when pressure is relieved. Plasmids are related to other genetic material as they also undergo the same process viz. replication, transcription etc and are transferred to their progeny i.e. it also inheritable.
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You isolate a piece of DNA from a microorganism you cultivated
from your teeth. The piece...
Directions: Read through each question thoroughly. through your assignment tab. Highlight the correct answer. Submit the quiz 1. Which enzymes are responsible for adding nucleotides to the DNA mo...
Directions: Read through each question thoroughly. through your assignment tab. Highlight the correct answer. Submit the quiz 1. Which enzymes are responsible for adding nucleotides to the DNA molecule during DNA replication? a) DNA replicase b) DNA polymerase c) DNA catalase d) DNA gyrase 2. A bacterial cell with faulty RNA polymerase would not be able to carry out a) DNA replication b) double helix formation c) DNA supercoiling d) transcription 3. R plasmids are of particular concern to humans...
What are SOS gene products? How are they induced? A. SOS proteins (RecA, SSB, uvrABC, etc);...
What are SOS gene products? How are they induced? A. SOS proteins (RecA, SSB, uvrABC, etc); nicked DNA binds to the Lac repressor, causing its release and degradation, thereby triggering expression of the SOS genes B. SOS proteins are the heat-shock proteins; the “chi” sequence binds to some transcription factors inducing expression of these genes C. SOS proteins are only transcription factors that activate other genes; DNA damage causes induction of these transcription factors through single-stranded DNA binding proteins D....
You isolate plasmid DNA from bacteria (Questions 7-10) 7) A plasmid is an extrachromosomal circul...
10 please and 7
You isolate plasmid DNA from bacteria (Questions 7-10) 7) A plasmid is an extrachromosomal circular DNA frequently found in prokaryotes. Aside from being smaller, how is it different from the prokaryotic genome? You place equal amounts of plasmid DNA in 4 different tubes and incubate the DNA with increasing amounts of the enzyme topoisomerase I for 1 hour (0 enzyme units 0.25 enzyme units, 0.5 enzyme units and 1 enzyme unit). You then analyze the plasmid...
Please solve Q4: Q1) This DNA sequence v is for an actual plasmid named pSB1A2-113521. It...
Please solve Q4:
Q1) This DNA sequence v is for an actual plasmid named pSB1A2-113521. It contains the 5' to 3' DNA sequence for constitutive production of a red fluorescent protein in bacteria, along with a ColE1 origin of replication and a resistance gene for the antibiotic ampicillin. The DNA is circular, so if one ran along the DNA and hit the end of the sequence, one would find themselves back at the beginning. The sequence of the complementary strand...
4. In a lake governed by the food web shown in the image to the right, 500 brown trout are added from fisheries. Which of the following outcomes would most likely be expected? A. Tadpole populat...
4. In a lake governed by the food web shown in the image to the right, 500 brown trout are added from fisheries. Which of the following outcomes would most likely be expected? A. Tadpole population will increase B. Duck population will decrease C. Algae population will increase D. Lilly Pad population will decrease E. Caddis Fly larvae will increase 5. Which of the following pieces of evidence most strongly supports a common ancestor of all life on Earth? A....
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteina...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving rise to a “hairless” phenotype. In the homozygous condition, H is lethal. An independently assorting dominant allele S has no effect on bristle number except in the presence of H, in which case a single dose of S suppresses the hairless phenotype, thus restoring the "hairy" phenotype. However, S also is lethal in the homozygous (S/S) condition. What ratio of hairy to hairless flies...
Need help filling in the chart and answering the questions that go along with it. I...
Need help filling in the chart and answering the questions
that go along with it. I have added the procedure and the
instructions as well as the "results" that are supposed to be used
to fill in the chart. Thank you!
We were unable to transcribe this imageTABLE 8-1 Cast of Characters and a Legend of Abbreviations Name Symbol Function in This Experiment Green fluorescent protein GFP It serves as an indicator of successful transformation and gene transcription expression in...
Directions: Read through each question thoroughly. through your assignment tab. Highlight the correct answer. Submit the quiz 1. Which enzymes are responsible for adding nucleotides to the DNA molecule during DNA replication? a) DNA replicase b) DNA polymerase c) DNA catalase d) DNA gyrase 2. A bacterial cell with faulty RNA polymerase would not be able to carry out a) DNA replication b) double helix formation c) DNA supercoiling d) transcription 3. R plasmids are of particular concern to humans...
10 please and 7
You isolate plasmid DNA from bacteria (Questions 7-10) 7) A plasmid is an extrachromosomal circular DNA frequently found in prokaryotes. Aside from being smaller, how is it different from the prokaryotic genome? You place equal amounts of plasmid DNA in 4 different tubes and incubate the DNA with increasing amounts of the enzyme topoisomerase I for 1 hour (0 enzyme units 0.25 enzyme units, 0.5 enzyme units and 1 enzyme unit). You then analyze the plasmid...
Please solve Q4:
Q1) This DNA sequence v is for an actual plasmid named pSB1A2-113521. It contains the 5' to 3' DNA sequence for constitutive production of a red fluorescent protein in bacteria, along with a ColE1 origin of replication and a resistance gene for the antibiotic ampicillin. The DNA is circular, so if one ran along the DNA and hit the end of the sequence, one would find themselves back at the beginning. The sequence of the complementary strand...
4. In a lake governed by the food web shown in the image to the right, 500 brown trout are added from fisheries. Which of the following outcomes would most likely be expected? A. Tadpole population will increase B. Duck population will decrease C. Algae population will increase D. Lilly Pad population will decrease E. Caddis Fly larvae will increase 5. Which of the following pieces of evidence most strongly supports a common ancestor of all life on Earth? A....
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...
Need help filling in the chart and answering the questions
that go along with it. I have added the procedure and the
instructions as well as the "results" that are supposed to be used
to fill in the chart. Thank you!
We were unable to transcribe this imageTABLE 8-1 Cast of Characters and a Legend of Abbreviations Name Symbol Function in This Experiment Green fluorescent protein GFP It serves as an indicator of successful transformation and gene transcription expression in...
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