Why is an exonuclease required for transformation of a naturally competent cell? Why is an exonuclease...
Why is an exonuclease required for transformation of a naturally competent cell? Why is an exonuclease NOT required for successful HGT via conjugation?
Homework Answers
1. Exonuclease breaks the DNA fragments, which are most effective, and separates them which can be further used for production of required proteins which will help survival even in normal cells which are competent.
2. HGT(horizontal gene transfer) via conjugation is a kindoff reproduction method in bacteria. It requires transfer of genetic material from one to another so it doesn't require exonuclease because no specific gene is required to be transferred.
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Why is an exonuclease required for transformation of a naturally
competent cell? Why is an exonuclease...
What is required for cells to be considered "naturally competent"? Electroporation Tra machinery Com machinery O...
What is required for cells to be considered "naturally competent"? Electroporation Tra machinery Com machinery O Heat shock
What process requires contact between the donor cell and the recipient cell? A. transformation B. transduction...
What process requires contact between the donor cell and the recipient cell? A. transformation B. transduction C. transcription D. conjugation
What process requires contact between the donor cell and the recipient cell? A. transformation B. transduction...
What process requires contact between the donor cell and the recipient cell? A. transformation B. transduction C. transcription D. conjugation
Please classify each statement as describing transformation, conjugation, or transduction in bacteria. Bacteria can acquire plasmids...
Please classify each statement as describing transformation, conjugation, or transduction in bacteria. Bacteria can acquire plasmids from outside the cell. A bacterium that contains an F plasmid connects to a recipient bacterium that lacks an F plasmid with an appendage called a pilus, through which the plasmid is transferred. Some bacterial DNA fragments may be included when new phage particles are assembled. A cell can be treated to make it competent to take up DNA from its environment. When a...
Answer the following questions: a) Explain why conjugation with an Hfr cell always results in the...
Answer the following questions: a) Explain why conjugation with an Hfr cell always results in the transfer of genes in the same order? (0.75 marks) b) Why does transformation result in only half of the progeny of the transformed cell carrying the alternate allele? (0.75 marks)
For the following please select the correct answer and explain why that answer is correct. 1)The...
For the following please select the correct answer and explain why that answer is correct. 1)The uptake of free DNA from the environment ________, while transfer of DNA with cell-to-cell contact would most likely result in ________. a) transformation / conjugation b)conjugation / transformation c) transformation / transduction 2) A deleterious mutation in recA results in: a) a decrease in homologous recombination. b) an increase in homologous recombination. c) no change in either general or specific recombination.
CLASS: Cell Biology (Biology 204) TOPIC: Bacterial transformation, purification, and recombination Why does the plasmid DNA...
CLASS: Cell Biology (Biology 204) TOPIC: Bacterial transformation, purification, and recombination Why does the plasmid DNA remain intact while the bacterial chromosome is sheared and subsequently lost during the miniprep procedure?
decribe why duplication mutations can sometimes be harmful to cell/organism and propose an example of how...
decribe why duplication mutations can sometimes be harmful to cell/organism and propose an example of how a duplication mutation can be beneficial evolutionary step for a cell/organism. Develop a rationale as to why eukaryotes have developed/evolved multiple mechanisms to repair DNA damage (polymerase 1 exonuclease activity, base excision repair, nucleotide excision repair mismatch repair, etc)
mechanisms of recombination from donor to recipient cell *kind of dna transfered (chromosome, plasmid, or both)...
mechanisms of recombination from donor to recipient cell
*kind of dna transfered (chromosome, plasmid, or both)
REVIEW Transformation Generalized transduction Conjugation F+ A. Kind of DNA transferred (chram oame, plamid or both) Is donor living or dead? Is direct contact required? yes or no Is a virus required? yes or no Will the process occur if DN Aase is in the medium? yes or no
number 2 Plasmid-small extrachromosomal dsDNA which contains genes that regulate non-essential life functions Transformation-acquired genes from...
number 2
Plasmid-small extrachromosomal dsDNA which contains genes that regulate non-essential life functions Transformation-acquired genes from free-floating DNA in the environment Competent cell-bacterial cell wall and cytoplasmic membrane become porous and allows transforming DNA to enter the cell 2. Explain why-control E. coli cells cannot grow on L.BA+amp and L.BA+amp+X-Gal. 3. goal: Name a procedure which can replace the heat shock process and achieve the same electroportation What in the ninnee of andinn amnicillin to I RA2
What is required for cells to be considered "naturally competent"? Electroporation Tra machinery Com machinery O Heat shock
Please classify each statement as describing transformation, conjugation, or transduction in bacteria. Bacteria can acquire plasmids from outside the cell. A bacterium that contains an F plasmid connects to a recipient bacterium that lacks an F plasmid with an appendage called a pilus, through which the plasmid is transferred. Some bacterial DNA fragments may be included when new phage particles are assembled. A cell can be treated to make it competent to take up DNA from its environment. When a...
Answer the following questions: a) Explain why conjugation with an Hfr cell always results in the transfer of genes in the same order? (0.75 marks) b) Why does transformation result in only half of the progeny of the transformed cell carrying the alternate allele? (0.75 marks)
decribe why duplication mutations can sometimes be harmful to cell/organism and propose an example of how a duplication mutation can be beneficial evolutionary step for a cell/organism. Develop a rationale as to why eukaryotes have developed/evolved multiple mechanisms to repair DNA damage (polymerase 1 exonuclease activity, base excision repair, nucleotide excision repair mismatch repair, etc)
mechanisms of recombination from donor to recipient cell
*kind of dna transfered (chromosome, plasmid, or both)
REVIEW Transformation Generalized transduction Conjugation F+ A. Kind of DNA transferred (chram oame, plamid or both) Is donor living or dead? Is direct contact required? yes or no Is a virus required? yes or no Will the process occur if DN Aase is in the medium? yes or no
number 2
Plasmid-small extrachromosomal dsDNA which contains genes that regulate non-essential life functions Transformation-acquired genes from free-floating DNA in the environment Competent cell-bacterial cell wall and cytoplasmic membrane become porous and allows transforming DNA to enter the cell 2. Explain why-control E. coli cells cannot grow on L.BA+amp and L.BA+amp+X-Gal. 3. goal: Name a procedure which can replace the heat shock process and achieve the same electroportation What in the ninnee of andinn amnicillin to I RA2
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