Question

If your specific activity of your enzyme is 20 micromoles of product/(min*mg protein), how many micromols of product can be attained in 2 minutes given 2 mg of protein?

a. 5 mol

b. 20 mol

c. 80 mol

d. cannot be determined from the data provided in the problem

Answer 1

The specific activity of the enzyme is 20 micromol per min per mg of protein.

That is in one minute, the enzyme will consume 1 mg of protein to produce 20 micromol of the product.

If we have 2 mg of protein, the enzyme will produce 20 X 2 = 40 micromol of product in 1 minute.

In 2 minutes, the enzyme will act on 2 mg of protein to produce 40 x 2 = 80 micromol of product.

Therefore, the answer is 80 micromol.