Add 1ml of culture to 999 ml of water. You will get 1:1000 dilution.
Or add 1µl of cultue to 999 µl of water. You will get 1:1000 dilution.
D= 1/(1+999)=1/1000=1 x 10-3 (this is usually written as 1:1000 dilution)
Explain how you would make a 1/1000 dilution of a bacterial suspension if you only had 3 ml aliquots of BHI broth (diluent) available. What size pipetman will you need to use to make this dilution?
A culture of E. coli is diluted 1:1000. 1 ml of the 1:1000 dilution is spread onto an agar plate. After incubation, 50 colonies are observed. What is the concentration of the original culture? Show your work and include proper units for full credit.
how would you prepare a 1:1000 dilution of a standard solution
A. You have been given a tube of E. coli. You are asked to make 1 mL total volume of 10-1 dilution of the bacterial culture. Explain how you would do this. Show all necessary calculations. ____ ml cells + _____ ml water = 1 ml (total volume) B. Next, you were asked to make a 10-2 dilution of the bacterial sample. Explain how you would perform this. Show all necessary calculations. You have bacteria at a concentration of 2...
The following details the results of a serial dilution of E. coli: Effective Dilution Factor on Plate CFUs 105 1095 106 178 107 5 108 None The "effective dilution factor on plate" already takes into account the fact that you plated 0.1 ml of the dilution. How many CFUs/ml were present in the original sample? a. 1.78 x 10^8 b. 1.14 x 10^8 c. 1.125 x 10^8 d. 1.095 x 10^8
How would you prepare 1.5 ml of a 1:1000 dilution of the CB2 primary antibody (1° Ab CB2) in 1% milk solution if 1.5 μl is provided in a tube. Specifically, how much 1% Milk in DPBS will you add to the tube?
How would you make 1 mL of a 1 to 25 dilution of yeast crude extract? Be specific.
How would you make 120 mL of a 1 to 25 dilution of a yeast extract? I'm really not understanding this process so steps/explanations would be nice. Thanks.
How would you make a 4 fold dilution of X-gal? What would the concentration of the diluted solution be?
Suppose your professor handed you a test tube with 2.0 ml of an E. Coli broth culture in it and told you to make a 10 -1 dilution of the entire culture. Explain howyou would do this. Show your calculations.